毕赤酵母表达系统是目前最为成功的外源蛋白表达系统之一。
The Pichia pastoris is one of the most successful foreign protein expression system until now.
结果经SDSPAGE和薄层扫描分析表明，外源蛋白表达量占菌体裂解蛋白总量的6 . 4 %。
Results SDS PAGE and thin layer scanning showed that the expressed product contained about 6.4% of total somatic protein.
要在一种宿主表达系统中成功表达外源蛋白并获得较高产量，必须要较为全面地了解影响其表达的诸多因素。
Numerous factors that influence heterologous protein production in a certain host system must be observed to gain successful gene expression and even with high yields.
为了进一步提高外源蛋白质的分泌表达，对信号肽序列进行了研究。
For further improving the secretion expression of heterogeneous proteins, in this research, the signal sequences were studied.
结论：为下一步在毕赤酵母中组成型表达外源蛋白，研究其作用机理和遗传机制奠定了基础。
Conclusion:The GAP promoter can be used to express foreign protein effectively and is the basis for studying the physicochemical properties and genetic mechanism of Gassericin T.
作者简述了利用植物生产外源蛋白的主要策略及异源基因在植物中表达的研究进展。
A brief review of major strategies for the production of heterologous proteins and the expression of foreign genes in plants is introduced in this article.
但对于一个特定的目标蛋白，选用何种受体植物更有利于外源蛋白的高效稳定表达和经济快捷的提纯，仍是需要首要考虑和解决的问题。
But for a special goal protein, it is the key question to choice the plant that can be in favor of foreign proteins' expressing and purifying.
外源基因在大肠杆菌中高效表达时，通常会形成不溶性、无活性的蛋白聚集体——包涵体。
Over expression of recombinant proteins in Escherichia coli cytoplasm often results in the formation of insoluble inclusion bodies.
结果剔除毕赤酵母中的PEP4基因后外源蛋白质表达量平均提高9．5%。
Results By disrupting the PEP4 gene in Pichia pastoris, the productivity of heterologous protein expression increased 9.5% on average.
这些技术策略的应用及改进将提高转基因在质体中特异有效表达，实现外源蛋白在质体中高水平积累。
The application and improvement of these systems will greatly enhance the specific and effective expression of the transgenes, and achieve high-level accumulations of foreign proteins in plastids.
结果：SDS - PAGE和薄层扫描分析表明外源蛋白的表达量占菌体裂解蛋白总量的18.6%。
Analysis of SDS-PAGE and thin layer scanning results showed that amount of expressed fusion protein was 18.6% in total lysis protein of bacteria.
这些作物在大田种植过程中以及收获后，其植物残体、根系分泌物和飘落的花粉等不断地进入土壤，可能造成外源基因表达蛋白在土壤中积累。
Transgenic resistant-pest plants can release transgene proteins through their residues, root exudates and pollens, and these proteins are bound rapidly to soil active particles and persisted in soil.
目前鸡输卵管生物反应器的研究集中在以卵清蛋白调控区来调控外源基因的表达。
At present, the research of chicken oviduct bioreactor focus on the expression of exogenous gene regulated by the ovalbumin 5 '-flanking regulatory region.
目前鸡输卵管生物反应器的研究集中在以卵清蛋白调控区来调控外源基因的表达。
At present, the research of chicken oviduct bioreactor focus on the expression of exogenous gene regulated by the ovalbumin 5 '-flanking regulatory region.
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