结论:可通过四环素抗性筛选系统筛选外源基因的高效表达克隆。
Conclussion: We could screen high expressed clone of heterologous gene using tetracycline resistance screening system.
PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性。
The results of PCR molecular detection, marker genes and foreign target-gene with insect-resistance were quite regular.
P CR检测结果表明,37株卡那霉素抗性植株中30株为阳性,说明vp7外源基因已整合到受体植物基因组中。
PCR detection results showed that 30 plants showed positive in 37 kanamycin resistance plants. Its indicated that the foreign gene VP7 had already integrated into genome of receptor plants.
研究结果表明:1导入不同外源基因的转基因抗虫棉因其杀虫机理不同而对棉铃虫抗性存在差异;
The result showed that: 1. These transgenic insect-resistant cotton after introducing different exogenous gene have different resistance to boll-worm because of distinct killing mechanism;
研究结果表明:1导入不同外源基因的转基因抗虫棉因其杀虫机理不同而对棉铃虫抗性存在差异;
The result showed that: 1. These transgenic insect-resistant cotton after introducing different exogenous gene have different resistance to boll-worm because of distinct killing mechanism;
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