目的：分离培养人脂肪基质干细胞，鉴定其表型。
AIM: To isolate and culture human adipose tissue derived stem cells, and identify their phenotype.
目的综述近年骨髓基质干细胞成软骨分化的研究进展。
Objective To review the recent research progress about the chondrogenic ability of bone marrow-derived mesenchymal stem cells.
免疫荧光法鉴定脂肪基质干细胞表达CD13和CD44。
The adipose stem cells expressed CD13 and CD44 through immunofluorescence.
目的：分离培养兔骨髓基质干细胞并探讨其体外生物学特性。
Objective To study the biological characteristics of rabbit bone marrow mesenchymal stem cells in vitro.
培养接种3周后，骨髓基质干细胞增殖较第二周无明显变化。
The conjunction of cells appeared about 1 week, and extended at 3 weeks, with a large amount of extracellular matrix around cells.
目的：探讨自体骨髓基质干细胞修复兔桡骨远端骨缺损的可行性。
OBJECTIVE:To explore the feasibility on repairing bone defect of rabbit radius by autologous bone marrow stem cells.
结论体外诱导骨髓基质干细胞定向分化为胰岛样细胞存在可行性。
Conclusion Functional islet-like cells can be differentiated from marrow mesenchymal stem cells, which may be a new procedure for clinical diabetes stem -cell therapy.
目的观察超低温冻存对人骨髓基质干细胞(BMSC)生物学特性的影响。
Objective To study the effects of cryopreservation on biological characters of human bone marrow stromal cell (BMSC).
方法：体外扩增诱导兔骨髓基质干细胞，将其与去细胞牛松质骨复合培养。
Methods Rabbit marrow stem cells (MSCs) were cultured and expanded in vitro. The MSCs were mixed with acellular bovine cancellous bone (ABCB).
目的：探讨温固化水凝胶作为骨髓基质干细胞的载体修复软骨缺损的可行性。
Objective To investigate the feasibility of the temperature dependent hydrogel loading bone marrow stromal cells for repairing articular cartilage defects.
重组病毒物理滴度测定后感染骨髓基质干细胞，在荧光显微镜下观察荧光强度。
After measuring virus titer, BMSCs were transfected and the fluorescence intensity was observed under fluorescence microscope.
前言：目的：研究体外培养的兔骨髓基质干细胞的成骨诱导及复合培养生长状况。
AIM: To study the induction of osteoblasts by rabbit bone marrow stromal stem cells (BMSSCs) and the growth status of the cells after co culture.
结论应用自体骨髓基质干细胞构建的组织工程肋骨在个体化治疗中显示了优越性。
Conclusion The tissue engineered bone constructed by autogeneic MSC is advantageous in individual treatment.
目的探讨一种较为有效的诱导方法将骨髓基质干细胞体外定向诱导分化为类许旺细胞。
Objective To search for a more efficient method to induce bone marrow stromal cells (BMSCs) into Schwann-liked cells (SLCs) in vitro.
超顺磁性氧化铁体外标记大鼠骨髓基质干细胞的传代：细胞中铁粒子会随传代而变化吗？
Passage of superparamagnetic iron oxide labeled rat bone marrow mesenchymal stem cells in vitro: Do iron particles change with passage?
就骨髓基质干细胞分离、诱导分化为软骨细胞的方法、载体材料及目前存在的问题加以综述。
In this article, isolation and differentiate regular of BMSCs, method of BMSCs induced to chondrocytes, scaffold of cartilage tissue engineering and problems of current existent were reviewed.
在微载体悬浮培养条件下，脂肪基质干细胞生长更为旺盛，细胞产量更高，优于常规静止培养。
Under the condition of microcarrier suspension culture, the adipose derived stem cells grew more productively, and the production of cell was higher, which were superior to those under static culture.
本实验主要利用骨髓基质干细胞的这一特性，对其进行成软骨和成骨诱导，建立诱导和鉴定体系；
This experiment mainly used this feature of bone marrow stromal stem cells to find ways to induce them into chondrocytes and osteoblasts, and also established the induction and appraisal system;
目的研究软骨分化形成蛋白- 2 (CDMP - 2)对小鼠骨髓基质干细胞软骨分化的作用。
Objective To study the cartilage differentiation of mouse mesenchymal stem cells (MSCs) induced by cartilage-derived morphogenetic proteins-2 (CDMP-2) in vitro.
目前已建立了体外培养骨髓基质干细胞的方法，而且正在摸索进一步纯化的方法和诱导分化的条件。
Cultivation, purification, as well as induction methods have been developed and that will definitely lead to further clinical application of the cells.
结果：成功地构建了重组BMP7逆转录病毒表达载体，并可在骨髓基质干细胞中表达BMP 7。
The viruses were used to infect directly BMSCs and the expression of BMP 7 gene in BMSCs was analyzed by immunohistochemical staining.
并在这基础上，把骨髓基质干细胞与生物支架材料复合培养，在体外构建工程软骨和软骨下骨修复体。
Then, on this basis, the bone marrow stromal cells were cultured with the scaffold biomaterials, and the engineering cartilage and subchondral bone restorations were constructed in vitro.
资料综合：骨髓基质干细胞具有多向分化能力，不具有特异性标志物，无法用某种特异性标志对其进行鉴定和分离。
DATA SYNTHESIS: The bone marrow mesenchymal stem cell had various ability of differentiation without specific sign, and could not evaluate and detach it with certain specific sign.
研究表明，骨髓基质干细胞作为一种具有多向分化潜能的干细胞，在适当的条件下可以被诱导分化成为软骨细胞和成骨细胞。
Studies have shown that bone marrow stromal cells, a kind of pluripotent stem cells, can be induced to differentiate into chondrocytes and osteoblasts under appropriate conditions.
将腺病毒感染的骨髓基质干细胞接种于修剪成一定形状的PLGA上，经电镜观察可见细胞贴附于材料表面和孔隙内并增殖。
Adenovirus-infected MSCs seeded onto PLGA showed high level of proliferation, and mass synthesis of cell matrix was observed with electronic microscoping.
结果所制备磷酸钙骨水泥主要成分为低结晶度的羟基磷灰石(HA)，对骨髓基质干细胞的生存、增殖能力及对其成骨特性的保存无影响。
Results the main component of CPC is hydroxyapatite (ha), which has no effect on the growth, proliferation, and capacity of bone forming of MSCs.
骨髓基质干细胞是一种由骨髓中分离获得的具有多种分化潜能的间质干细胞。在体外培养条件下，它可以分化为成骨细胞、软骨细胞、脂肪细胞、甚至于成肌细胞。
Bone marrow stromal stem cells isolated from bone marrow are the special mesenchymal stem cells, which can be differentiated in culture into osteoblasts, chondrocytes, adipocytes, and even myoblasts.
目的：探讨骨髓基质细胞条件培养液诱导中脑神经干细胞分化为高比例神经元的机制。
Objective: To investigate the effects of bone marrow stromal cell conditioned medium on differentiation of mesencephalic neural stem cells into neurons.
目的以原代培养小鼠骨髓基质细胞为饲养层，探讨精原干细胞能否在该饲养层上生长及生长的影响因素。
To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.
目的以原代培养小鼠骨髓基质细胞为饲养层，探讨精原干细胞能否在该饲养层上生长及生长的影响因素。
To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.
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