目的选择合理的标本处理方法,提高外周血基因转录本检测的灵敏度。
Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.
本研究的GPBF基因为玉米中首次报道的GA合成相关转录因子。
GPBF is the first report of transcriptional factor of GA biosynthesis-related in maize.
在这些标本中,我们首先对20,661蛋白编码基因的23,219转录本进行了测序。
We first determined the sequences of 23,219 transcripts, representing 20,661 protein-coding genes, in these samples.
这说明它们是一个基因的两个转录本,使用了不同的转录起始位点。
It hints that HSEI and HSEII are two transcripts of HSE gene transcribed from the different transcription start sites.
到每个转录本或基因的比对片段的数目在不同条件和重复下变化。
The number of mapped reads to each transcript or gene varies under different conditions and replicates.
本研究的目的是从基因的转录和翻译水平对此进行验证。
In this study, we testified the alteration of desmuslin expressing style at gene transcription and translation levels.
本研究工作集中于面包房酵母的DNA转录问题,主要是因为该有机物的基因组业已得到广泛的研究。
The research focused on DNA transcription in baker's yeast, largely because that organism's genome has been extensively studied.
该基因存在多种不同的转录本,而RASSF1A是最重要最常见的转录本。
RASSF1 consists of many variants, of which RASSF1A is the most important and commonly.
实验证明,此基因的转录是由低温诱导激活,且随着雪莲低温驯化时间的推移,转录本的量明显增加。
Our experiment showed that this gene was induced by low temperature and there was an increase in the level of its transcript during cold acclimation.
在外显子水平,共有85个发生选择性剪接的外显子,分别属于85个转录本,仅有36个已知基因。
On exon level, 85 PSRs (probe select regions) were identified as alternatively spliced exons that belonged to 85 ASTs (alternatively spliced transcripts), and 36 transcripts have an-notation.
本实验采用定量反转录聚合酶链式反应技术,观察大鼠在进行11周中等强度耐力训练过程中心脏心钠素(ANP)和脑钠素(BNP)基因表达的变化。
This study adopted RT-PCR technique to observe the change of express of gene of rats' ANP and BNP during 11 week's moderate intensity endurance training;
本实验采用定量反转录聚合酶链式反应技术,观察大鼠在进行11周中等强度耐力训练过程中心脏心钠素(ANP)和脑钠素(BNP)基因表达的变化。
This study adopted RT-PCR technique to observe the change of express of gene of rats' ANP and BNP during 11 week's moderate intensity endurance training;
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