在现代分子生物学技术研究中，常常需要对已知位点的侧翼序列进行分析或克隆，以研究基因的遗传表达调控。
In modern research of molecular biology, we usually need to analyse or clone these flanking sequence in given sites, so as to study gene expression and control.
并将外源基因alb亚克隆入该调控序列内，转染c 127细胞，经免疫组化法检测其表达情况。
Subclone the exogenous gene ALB into the regulating sequence for transfection of C127 cells.
在真核细胞，尽管一些转录活性基因和转录沉默基因的调控序列位置常常非常接近，它们的表达却并不互相影响。
In the eukaryotic cells, despite how close the regulation elements of active and inactive genes are frequently, they dot not affect each other expression level.
因此选择对基因的表达具有重要功能的外显子1和其上游的调控区及下游外显子内含子交界区序列通过直接测序法进行SNP筛查，并进一步分析多态位点与病窦的关联性。
Therefore the other aim of our study is to discover SNPs of HCN4 exon 1, including the regulatory sequence and exon-intron boundary of it, which plays an importable role of channel function.
因此选择对基因的表达具有重要功能的外显子1和其上游的调控区及下游外显子内含子交界区序列通过直接测序法进行SNP筛查，并进一步分析多态位点与病窦的关联性。
Therefore the other aim of our study is to discover SNPs of HCN4 exon 1, including the regulatory sequence and exon-intron boundary of it, which plays an importable role of channel function.
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