构建基因组文库是一项非常基础和重要的工作。
基因组文库是进行基因组研究的基础工作之一。
The bacterial artificial chromosome (BAG) library is the basic tool for the genome research.
宏基因组文库技术是利用未培养微生物基因资源的有效途径。
The metagenomic library-based technique is an effective strategy for the utilization of un-cultured microorganisms.
但利用传统方法构建基因组文库存在操作步骤繁琐、背景高等问题。
Because traditional methods of constructing genomic libraries are very fussy and produce high background, we improve the traditional methods to solve these disadvantages.
基因组文库中分离到一个与人类肿瘤抑制基因QM具有同源性的基因，命名为OSQM1。
A gene homologous to the human putative tumor suppressor gene QM, designated OSQM1, was isolated from rice Oryza sativa l.
对土壤宏基因组DNA的提取、宏基因组文库的构建和筛选等方面的研究现状和进展进行了简要综述。
Progress on the research of metagenomic DNA purification, metagenomic library construction and screening of soil microorganisms are reviewed briefly.
细菌人工染色体(BAC)是一种承载dna大片段的克隆载体系统，用于人、动物和植物基因组文库构建。
Bacterial artificial chromosome (BAC) is a kind of vector system used to construct large fragment insert libraries of genome DNA in human, animals and plants.
结合不断完善的基因组文库信息，他们利用数学模型模拟了基因的演变，他们发现，基因家族是可以不断继承、延续的；
They combined information from the ever-growing genome library with their own mathematical model that takes into account the ways that genes evolve: new gene families can be born and inherited;
克隆文库组成分析、分子杂交和群落指纹图分析被用来分析微生物群落的基因组信息组成。
Clone library profiling, molecular hybridization and community fingerprinting have been used to retrieve genomic information in a community.
以高通量基因组序列(HTGS)数据库及SAGE文库为基础，进行染色体定位及组织表达分析。
Bioinformatic data of High Throughout Genomic Sequences (HTGS) and Serial analysis of Gene expression (SAGE) were used for analysis of chromosome localization and tissue expression.
结论：所构建的基因组d NA消减文库为进一步筛选UPEC132中与致病相关基因奠定了基础。
Conclusion: The DNA subtractive library may provide a solid foundation for screening and cloning pathogenic genes of UPEC132.
用吸附后的血清通过菌落原位免疫杂交筛选该基因组表达文库，从3000个菌落中获得了12个阳性克隆。
The genomic expression library was probed used the adsorbed-pooled sera, and 12 positive clones were obtained from 3000 postulating colonies by immunological hybridization.
细菌人工染色体(BAC)文库是复杂基因组生物的基因组研究中最方便、最可靠、应用最广的文库。
Bacterial artificial chromosome (BAC) libraries are the most convenient, reliable and widely used genomic libraries in genome research of organisms with highly complex genomes.
鸟枪法构建VP3噬菌体全基因组随机文库；
A random library of VP3 DNA was constructed by shot-gun library method.
该方法为进一步构建中棉所12号多倍基因组的BAC文库、从而进行棉花基因组有关研究奠定了基础。
The method will lay a foundation for more-genome-equivalent BAC library construction of CCRI 12 and cotton genome research.
着重论述了宏基因组学的研究方法，包括DNA的提取、文库的构建以及筛选策略的选择。
Methods in metagenomic study, including DNA extraction, library construction and screening were discussed in this paper.
进一步表明，上述的三个条件完全符合构建棉花线粒体基因组细菌人工染色体文库的要求。
Further indicated that, the above three conditions completely conform to the construction cotton mitochondrial DNA bacterial artificial chromosome library requirement.
目前，在基因组范围内富集基因的方法有cD NA文库、甲基化过滤文库、高Cot值文库、转座子标签富集法等。
Currently, several approaches have been developed for gene enrichment on a genome-wide scale, such as cDNA library, methylation filtration library, high Cot library and transposon tagging.
目前，在基因组范围内富集基因的方法有cD NA文库、甲基化过滤文库、高Cot值文库、转座子标签富集法等。
Currently, several approaches have been developed for gene enrichment on a genome-wide scale, such as cDNA library, methylation filtration library, high Cot library and transposon tagging.
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