为研究IBV变异株的基因组及其变异特征，本实验通过基因扩增拼接的方法获得了肾病变型毒株JH06111的全基因组序列。
To study the genomic characteristics of IBV variant isolate, we obtained the full genome sequences of nephropathogenic JH06111 by amplifying, cloning and sequencing of its cDNA.
最终，研究人员获得40倍的基因组的覆盖面和运用 454GS 的De Novo序列拼接软件（Newbler）绘制出了高质量的草图。
In the end, the researchers obtained 40x genome coverage and generated a high-quality draft assembly using the 454 GS De Novo Assembler Software (Newbler).
最终，研究人员获得40倍的基因组的覆盖面和运用 454GS 的De Novo序列拼接软件（Newbler）绘制出了高质量的草图。
In the end, the researchers obtained 40x genome coverage and generated a high-quality draft assembly using the 454 GS De Novo Assembler Software (Newbler).
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