基因表达具有明显的多尺度特征，分类率最大达到98.61%，结果稳定。
There are multi -scale feature gene expression, the maximum classification rate is 98.61%, with stable results.
新的转化程序要求把基因导入农艺性状优良的品种中，呈单拷贝、不带有辅助序列，并在不同的转化体中表达一致，稳定遗传。
Transgenes should be with single copy and without accessory sequence, and with consistence to express and stable genetic in various transgenic plants in a new gene transformation program.
结果：建立了新基因HBRP稳定表达的基因工程细胞系。
Results: the stable expression cell system of the novel gene HBRP was constructed.
结论：利用脂质体介导的基因转移方法介导骨髓细胞基因转移可获得高效、稳定的表达。
Conclusion: Liposome mediated gene transfer in bone marrow cells is highly efficient and the expression of the transferred gene is stable.
外源基因在大肠杆菌染色体上的稳定表达并不影响细菌的生长繁殖。
The stable expression of exogenous gene in E. coli chromosome had no effect on the bacterial growth and propagation.
结论构建了含人pd - 1基因重组逆转录病毒的载体，筛选出稳定表达人PD - 1分子的L929细胞株。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
表明第7染色体上位于标记R1357~R1245之间的种子休眠性QTL位点是一个在年度之间稳定表达的控制种子休眠性的基因位点。
The QTL for seed dormancy on chromosome 7 between the marker R1357 and R1245 was a stable expressional locus associated with seed dormancy in different years.
这些结果证明供体燕麦上可能携带抗小麦条锈菌的新基因，并已通过花粉管通道进入小麦，整合到小麦的染色体组中，稳定表达。
The result also indicated that the new genes resistant to yellow rust may be carried on oat DNA, which have been integrated into the genome of wheat and expressed steadily.
近年来，很多研究者发现上述持家基因的表达水平并不稳定，利用它们作为内标来定量并不准确。
But in recent year, many researchers observed that the expression of housekeeping gene was not stable at all conditions.
前病毒整合位点、靶细胞特性和整合后的载体结构与转入基因表达的稳定性紧密相关。
The integration sites of provirus, the nature of the target cells and the structure of vectors were closely related to the stability of gene expression.
新的基因转化程序要求转基因为单拷贝，不带有标记基因，并在不同的转化体中表达一致，稳定遗传。
Transgenes should be genes with single copy and without marker gene, and be able to express and stable inheritable in various transgenic plants in a new gene transformation program.
为满足转目的基因的需求，探索一种能够将外源基因转入银杏细胞，并得以稳定表达和遗传的转基因方法以及选择合适的载体。
To meet the demands of gene transfer of an objective gene, a suitable vector should be screened to carry the extraneous objective gene into the callus cell of Ginkgo biloba.
目的为建立能稳定表达人葡糖醛酸转移酶UDPGT1A9蛋白的CHL UDPGT1A9转基因细胞系，并鉴定其对药物的葡糖醛酸缀合活性。
AIM To establish a cell line CHL UDP glucuronosyltransferase gene (UDPGT1A9) which will stably express human UDPGT1A9 protein and determine the activity of expressed UDPGT1A9 in drug glucuronidation.
型核纤层蛋白决定细胞核的大小及形状，与细胞核的稳定性、染色质结构以及基因表达有关。
Lamin proteins are thought to determine the size and shape of cell nuclei and to be involved in nuclear stability, chromatin structure and gene expression.
在HCV的编码基因中，核心区(C区)序列具有高度遗传保守性，c基因表达产物具有良好的抗原稳定性。
Among HCV encoding gene, core region shows greater sequence conservation. Core gene expression has favorable antigen stability.
砷可能通过抑制砷中毒患者皮肤组织中MGMT、XRCC1等DNA修复基因的表达，影响基因组d NA稳定性和DNA修复功能而导致对皮肤的致癌作用。
Arsenic causes carcinogenicity on human skin through inhibiting the expressions of MGMT, XRCC1 and influencing the genetic stability and the DNA repair function.
结果单、双自杀基因均在GLC- 82细胞中稳定表达，双基因转染组对细胞增殖的杀伤及旁杀伤效应高于单基因组。
Results Double and single suicide gene transfer were both stably expressed in GLC-82 cells. The cytotoxic effects of co-expressed TK-CD genes were superior than that of the single gene.
不同材料间具有稳定表达的组成型RRMRNA结合蛋白基因，同时具有丰富的差异表达的具调控功能的RRM RNA结合蛋白基因。
The primer was designed based on consensus sequence from RRM RNA binding protein gene family. The results showed that there were stable expressed fragments among the different mRNA samples.
结论成功构建了带有大鼠bmp - 7基因的慢病毒载体，并实现其在HSC - T6的稳定高表达。
Conclusion Lentiviral vector carrying BMP-7 gene has been successfully constructed and maintains high expression in HSC-T6 cells.
实时荧光定量PCR结果表明，该基因在受到条锈菌诱导下，在亲和组合中表达趋势明显下调，而在非亲合组合中的表达趋势比较稳定。
The results of real-time PCR analysis revealed that TAFFC transcripts were steady in incompatible interaction of wheat and stripe rust, whereas down-regulated in compatible interaction.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
结果：外源基因转移后14天表达尚很稳定，治疗基因在受体关节的滑膜衬里部位表达。
Results Assay of joint lavages confirmed the in vivo expression of biologically active hIL1Ra and hIL-10 and gene expression was not lost 14 days after transfer.
结论：稳定表达人ox40l的乳腺癌转基因细胞在体外能有效地活化t细胞，介导其增殖、分泌细胞因子及抑制T细胞活化诱导的细胞死亡。
CONCLUSION: OX40L-MDA-MB-435 cells could activate t cells in vitro, promote t cell proliferation and cytokine secretion, and suppress t cell activation-induced cell death.
结论：稳定表达人ox40l的乳腺癌转基因细胞在体外能有效地活化t细胞，介导其增殖、分泌细胞因子及抑制T细胞活化诱导的细胞死亡。
CONCLUSION: OX40L-MDA-MB-435 cells could activate t cells in vitro, promote t cell proliferation and cytokine secretion, and suppress t cell activation-induced cell death.
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