目的：研究建立等位基因特异性引物pcr技术体系，并将其应用于基因单核苷酸多态性研究工作。
Objective: to study and establish an allelic specific primer polymerase chain reaction (ASP-PCR) technique system and to apply this technique to study single nucleotide polymorphism (SNP) of genes.
目的：(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法：采用序列特异性引物聚合酶链反应的方法对汉族72例uc患者和314例正常对照者HLA - DRB1基因分型。
Methods: By using polymerase chain reaction-sequence specific primers, frequency of HLADRB1 alleles in 72 patients with UC and 314 healthy controls were detected.
方法采用多对型特异性引物-聚合酶链反应检测160例慢性乙型肝炎患者血清HBV基因型；
Methods: Serum samples from 160 cases with chronic HBV infection were collected and tested for HBV genotypes by type-specific primers.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法4例因献血感染HCV病人的系列血标本，用特异性引物扩增5'-NCR和核心区基因并进行测序。
Methods Serial sera were collected from 4 plasma-donors with HCV infection. 5' - NCR and core gene were amplified, sequenced and analyzed using software.
该研究通过设计合成hla基因序列特异性引物，建立了HLA -DR基因分型的套式扩增和直接扩增pcr -SSP技术，并在骨髓移植配型中进行了应用。
In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.
根据已报道的马铃薯卷叶病毒基因组序列，设计合成一对特异性引物。
Based on the reported genomic RNA sequence of PLRV, two specific primers were synthesized.
引物的特异性和灵敏度采用基因型已知的质控DNA进行验证。
Control DNA samples that genotypes known were used to confirm the sensitivity and specificity of each sequence-specific primer.
方法提取患者脑脊液和血清中的病毒rna，用肠道病毒特异性引物进行套式rt - PCR扩增，检测特异性基因片段。
Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.
采用顺序特异性引物聚合酶链式反应（PCR-SSP）DNA分型技术，首次对35例肾移植供受者和4份标准DNA进行HLA-DR2、DR7、DR9基因配型。
HLA-DR2, DR7, DR9 genotyping by polymerase chain reaction with sequence-specific primers (PCR-SSP)was carried out for 35 individuals and 4 cell lines DNA.
结论：本研究成功建立了一种改良hbv型特异性引物pcr基因分型方法，其具有灵敏、准确、快速、经济、国内适用等特点。
Conclusion: The study has successfully established a sensitive, accurate and fast, suitable for domestic application HBV genotype-specific primers PCR typing method.
在对OPF0 2 757进行克隆、测序的基础上，设计一对PCR引物，建立了簇毛麦基因组特异性PCR标记。
On the basis of cloning and sequencing of OPF02 757 , two PCR primers were designed and a genome specific PCR marker for H.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA，采用RSCA和序列特异性引物体外基因扩增(PCRssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA，采用RSCA和序列特异性引物体外基因扩增(PCRssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
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