综述了T-DNA标签法的特点及其在水稻功能基因组研究中应用的进展。
The features of T-DNA tagging and the progress of its application in the research of rice functional genome were reviewed in the paper.
转座子标签法是克隆与分离植物基因的一项十分有效的方法。
Transposon tagging is an effective method for plant gene cloning and isolation.
结论表达序列标签法、步移法测序和生物信息学技术有利于发现日本血吸虫新基因。
Conclusion It is a feasible method to discover and acquire novel genes of S. japonicum by ESTs, combination of primer-walking sequncing and bioinformatics technique.
以往的基因克隆方法如图位克隆法、转座子标签法等大都是建立在正向遗传学的基础上，这些方法只适用于一个或少数基因，应该说是零敲碎打的策略。
However the former cloning methods such as map-base cloning or transposon tagging which based on forward genetics can only cloned one or several genes at the same time.
T - DNA标签法转化植物建立突变体库是植物功能基因组研究的重要而有效的方法。
Using T-DNA tagging approach for transforming plant is a powerful tool for the study of plant functional genome.
目前，在基因组范围内富集基因的方法有cD NA文库、甲基化过滤文库、高Cot值文库、转座子标签富集法等。
Currently, several approaches have been developed for gene enrichment on a genome-wide scale, such as cDNA library, methylation filtration library, high Cot library and transposon tagging.
目前，在基因组范围内富集基因的方法有cD NA文库、甲基化过滤文库、高Cot值文库、转座子标签富集法等。
Currently, several approaches have been developed for gene enrichment on a genome-wide scale, such as cDNA library, methylation filtration library, high Cot library and transposon tagging.
应用推荐