研究结果为鸭GAPDH作为内参基因用于鸭相关基因定量表达分析奠定了基础。
The results of this study will provide basis for GAPDH used as a reference gene to analyze the duck gene quantitatively.
三种新的基因位点与人眼颜色的微小及定量变化有关。
Three new genetic loci have been identified with involvement in subtle and quantitative variation of human eye colour.
这意味着研究者不但可以定性定量地分析肠道菌种，也可以这些菌种是通过那些基因对不同的环境条件进行应答的。
That means researchers could determine not only which species of bacteria were present and in what proportions, but also which genes these bugs were actively using in different conditions.
本发明涉及一种可制作标准曲线的临床定量检测基因芯片方法，属于生物技术领域。
The invention relates to a method of the clinical gene chip quantitative measurement for manufacturing a standard curve, and the method belongs to the biological technology field.
定量PCR结果表明该基因的表达模式与所选取的抗性及抗性相关基因的表达模式一致。
Quantitative RT-PCR results indicated representation of OsBTB gene expression was corresponding with the selected resistant and pathogenesis-related genes.
最后，用定量实时PCR法在一组新样本(20名发作性睡病-猝倒患者和20名健康对照)中进一步确认候选基因的表达水平。
Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy patients and 20 healthy controls).
这些结果表明，新的相对定量方法是一种简便、准确和高效的定量基因表达的方法。
The results show that the method described here is simple, precise and cost-effective for relative quantifying gene expression.
首次提出并实现了一种高灵敏度、定量的电化学发光特征基因检测方法。
For the first time and achieved a high sensitivity, the quantitative characteristics of the electrochemiluminescence gene detection method.
方法采用半定量pcr方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。
Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.
目的探讨胰腺癌细胞（PC-3）胰岛素样生长因子-1受体（IGF- 1R）基因的定量表达，及其与细胞凋亡、成瘤性的关系。
Objective To explore Insulin like growth factor 1 receptor(IGF 1R)gene quantitative expression in human pancreatic cancer cells(PC 3) and the relationship of IGF 1R with apoptosis?tumorigenicity.
同时，本发明的方法可将基因芯片的高通量检测与定量检测结合，扩大基因芯片的应用范围。
Simultaneously, the method of the invention can combine the high flux measurement and the quantitative measurement of a gene chip, and the application range of the gene chip is enlarged.
结果：荧光定量方法可以快速完成MTHFR C677T基因多态性的检测，检测结果得到传统PCR-RFLP方法的证实。
Results : Detection of MTHFR gene C677T polymorphism was quickly finished by realtime fluorescence quantitative RT - PCR, and the results were proved to be effective by PCR - RFLP.
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
为了识别、定量和注释所有的人类蛋白质编码基因，HPP将使用三种工具：质谱、亲和抗体、以及生物信息学。
To identify, quantify and annotate all human protein-coding genes, the HPP will use three tools: mass spectrometry, antibody affinity and bioinformatics.
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
经生物素标记探针杂交确认RANTES基因的整合，用免疫斑点印迹法对重组基因表达水平进行定量测定。
The integration of RANTES gene was confirmed by hybridization with biotin-labelled probes and the recombinant gene expression level was quantitatively detected by immunodot blotting.
干预：40只大鼠，于伤后不同时间取水肿区脑组织及正常脑组织：提取组织总rna：用共扩增反转录定量pcr方法测定MMP - 9基因表达水平。
INTERVENTIONS: Tissues of edema area and normal brain of 40 rats were collected in different time after damage: total RNA were abstracted and MMP-9 gene expression was tested by PCR means.
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
蛋白质组学研究直接定位于蛋白质水平，从整体、动态、定量的角度去研究基因的功能，是后基因组计划的一个重要组成部分。
Proteomic research is to reveal the function of genes from an integrated, kinetic and quantitative view at the global protein level, which is an important component of post genome project.
对筛选出的部分差异基因，选取病例组18例及正常对照组6例，以实时荧光定量PCR进行验证。
To filter out some of the differential genes, 18 cases of AS group and 6cases of healthy control group are validated by real-time fluorescence quantitative PCR.
方法利用寡核苷酸基因芯片技术和实时定量pcr技术检测20例胰腺癌组织和6例正常胰腺组织中TUSC3基因的表达。
Methods The expression of TUSC3 gene in 20 cases of pancreatic cancer and 6 cases of normal pancreas were detected by oligonucleotide microarray and real-time quantitative PCR.
近年来，差异显示技术也被应用于定量分析环境刺激对于细菌基因表达的影响。
In recent years DD also has been adapted to quantitative environmental stimuli on bacterial gene expression.
行为遗传学的发展前景将是定量遗传学和分子遗传学以行为基因组学为中心的整合。
The future for behavior genetics is integration of quantitative genetics and molecular genetics centering on behavior genomics.
在这两个基因表达的定性研究之后，还对这两个基因的表达进行了定量研究。
After qualitative study, there was a quantitative study on these two genes' expression.
应用逆转录PCR结合同位素定量分析，对32例儿童白血病患者的多源耐药基因表达水平进行了研究。
Quantitative RT PCR method were used to detect the mRNA levels of multidrug resistance gene in 32 leukemia children.
建立了玉米转基因成分中35s启动子非同源模板的竞争定量pcr检测系统。
The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35s promoter heterologous template for the detection of genetically modified maize was developed in this study.
利用该标准质粒建立的定量方法测定了我国12种白叶枯菌株基因组中IS1112的拷贝数。
The copies of IS1112 in twelve Xoo strains from China were identified using realtime quantitative PCR with this standard plasmid.
近年来，很多研究者发现上述持家基因的表达水平并不稳定，利用它们作为内标来定量并不准确。
But in recent year, many researchers observed that the expression of housekeeping gene was not stable at all conditions.
近年来，很多研究者发现上述持家基因的表达水平并不稳定，利用它们作为内标来定量并不准确。
But in recent year, many researchers observed that the expression of housekeeping gene was not stable at all conditions.
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