从中确定候选基因七个，利用电子延伸技术获得候选基因全长或5’、3’端。
Full-length or 3'and 5'sequences of seven genes were forecasted by electronic elongation.
结论：成功克隆SARS冠状病毒核衣壳蛋白基因全长，并在酿酒酵母中诱导表达成功，有助于抗sars分子疫苗的研究。
Conclusion Successful clone of an integrity SARS-CoV nucleocapsid gene and its effective expression in saccharomyces cerevisiae can be beneficial to the research on SARS vaccine.
目的：表达人免疫相关鸟苷三磷酸酶基因(IRGM) a全长融合蛋白，制备高质量的兔抗人IRGM多克隆抗体。
Aim: To express the whole length fusion protein of human IRGMa and prepare high quality rabbit anti-human immune-related gene guanosine triphosphate (IRGM) polyclonal antibody.
PNZIP基因启动子是一个活性很高的启动子，全长启动子在叶片的启动活性是35S启动子在叶片启动活性的9倍。
The full-length promoter of PNZIP gene is a strong promoter whose GUS activity in leaves is 9 times as that of 35S.
目的探索新克隆的编码人蛋白翻译起始因子的全长基因（C2基因）对细胞周期的影响及与细胞凋亡的关系。
Objective To investigate the effect of a new cloned full length gene, C2 gene which encodes a translation initiation factor, on cell cycle and the relationship between C2gene and apoptosis.
目的探索新克隆的编码人蛋白翻译起始因子的全长基因(C2基因)对人胃癌细胞生长特性的影响。
Objective to investigate the effect of a newly cloned full length gene, C2 gene, which encodes human eukaryotic translation initiation factor, on growth of gastric cancer cells.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
采用聚合酶链式反应（PCR）技术对来自北京等地区的173份PCV2阳性样本的全长ORF2基因进行了扩增，并对扩增产物进行了限制性片段长度多态性（RFLP）分析。
Whole ORF2 genes of 173 positive clinical PCV2 samples from Beijing and other areas were amplified by PCR and followed by restriction fragment length polymorphism (RFLP) analysis.
中国科学院国家基因研究中心率先完成了国际水稻基因组计划的第4号染色体全长序列的精确测序，该成果已在英国的《自然》杂志发表。
As part of international efforts to completely sequence the entire rice genome, national Center for Gene Research has finished the sequence and analysis of rice chromosome 4.
目的分析不同刺激下外周血单个核细胞(PBMC)中THANK基因的表达，并克隆全长的人THANK基因。
Aim to analyze THANK gene expression in peripheral blood mononuclear cells (PBMC) stimulated with different stimulators and to clone whole-length human THANK gene.
成功获得了人BMP4的全长基因，且序列与已知序列高度一致。
The full-length of human BMP4 is obtained successfully and its sequence is highly consistent with the known sequence.
目的：构建丙型肝炎病毒(HCV)全长及3种不同缺失突变的核心蛋白基因的原核表达载体，并在大肠杆菌中表达。
AIM: to construct the recombinant plasmids expressing full-length HCV core protein gene and 3 different deletion mutated hepatitis core protein genes and to express them in E. coli.
在国内外首次利用抗体探针获得了植物抗病蛋白编码基因的部分序列，为进一步克隆全长的API蛋白基因奠定了基础。
It is the first report of obtaining partial sequences of plant disease resistance gene by using antibody probe in the world, which provides a basis of cloning the whole gene encoding API protein.
将其全长片段与GUS基因连接构建植物表达载体并转化烟草。
A plant expression vector was then constructed by ligating the cloned fragment to GUS gene, used for transformation of tobacco.
目的：克隆测序新分离呼肠病毒的S1全长基因组片段，并对其进行序列分析。
Objective: To clone, sequence and analyze genome segment 1 (S1) of new isolated reovirus strains.
膜受体是一类膜蛋白，而全长的膜蛋白很难通过基因工程获得，这给制备该类蛋白的抗体增加了难度。
Membrane receptors belong to a kind of membrane proteins, the full length protein of which is hard to be expressed in vitro.
目的：克隆大鼠神经营养因子4全长基因，构建真核细胞表达质粒。
AIM: to clone rat neurotrophin-4 (NT-4) total gene and construct expression plasmid for prokaryotic cells.
而昆虫p 450基因序列已克隆的全长序列中大部分属于CYP4和CYP6家族，两个家族成员分别占总数的20%和45%。
Of the total full-length sequences of P450 genes of insects registered, 20% are CYP6 members and 45% CYP4 members.
结果从活化的人白细胞中克隆到IDO基因编码区全长，并构建了IDOEGFP融合蛋白表达载体。
Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
目的对命名为F蛋白结合蛋白1（FBP1）的未知基因，克隆其全长基因并进行生物信息学分析。
Objective To clone and bioinformatically analyze the full-length sequence of F protein binding protein 1 (FBP1).
电子克隆与其他发现全长基因的方法相比可以充分利用已有的数据库资源，避免大量的重复性劳动，节省时间和开支，加快新基因的发现。
Compared with other ways, this technology makes best use of existing database to avoid much of repetitive work, and saves time and expenditure, which makes it more rapid to find unknown genes.
方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。
Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
在一项具体实施例中，全长的合成基因组是由化学合成的核酸组分或者其副本构建而成。
In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components.
在一项具体实施例中，全长的合成基因组是由化学合成的核酸组分或者其副本构建而成。
In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components.
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