人血管生成素-1基因的克隆及表达载体的构建，为进一步研究其功能、活性和应用奠定了基础。
The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.
目的构建HP450基因的克隆载体。
结果：将编码人单核细胞趋化蛋白—1（MCP—1）基因克隆至融合表达载体pGEX—2T中，DNA测序证实正确。
Results: A gene fragement encoding MCP-1 was cloned into the fusional expression vector PGEX-2T. DNA sequencing indicated that it was correct.
目的克隆大鼠水通道蛋白4（AQP4）M23基因并构建其载体为进一步研究提供理论基础和研究工具。
Purpose: AQP4 M23 gene cloning and construction in the expression vector can provide us a research tool.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
结果从活化的人白细胞中克隆到IDO基因编码区全长，并构建了IDOEGFP融合蛋白表达载体。
Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
细菌人工染色体(BAC)是一种承载dna大片段的克隆载体系统，用于人、动物和植物基因组文库构建。
Bacterial artificial chromosome (BAC) is a kind of vector system used to construct large fragment insert libraries of genome DNA in human, animals and plants.
目的克隆人TRAIL基因，构建其原核表达载体。
To clone gene of the TRAIL and construct its prokaryotic expression vector.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。
This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.
目的：克隆人和小鼠的活化t细胞表达与分泌调节基因(RANTES基因)并分别构建腺病毒表达载体。
Objective: to clone the human and murine origin RANTES genes and construct the adenoviral expression vectors.
方法构建克隆1个含有JAK2基因和二聚化化学诱导物(AP2 0 187)结合位点所组成的逆转录病毒载体。
Methods a retrovirus vector (RV) which contains JAK2 gene and two binding sites for a chemical inducers of dimerization (AP20187) was constructed.
目的建立风疹病毒包膜糖蛋白e1的克隆载体；研究e1基因变异情况，并对其序列进行系统发生树分析。
Objectives to construct the cloning vector of glycoprotein E1 gene of rubella virus, to study the mutation of glycoprotein E1 gene, and to analyze the sequence of E1 gene by the phylogenetic tree.
该启动子的成功克隆和其报告基因载体的构建，为研究SOD2的基因表达调控机制提供了重要基础和工具。
The reporter gene vector driven by SOD2 promoter will provide an experimental tool for the further study on the regulatory mechanism of the SOD2 expression.
本研究克隆了苹果NAD-SDH 基因的部分cDNA 序列，构建了其反义表达载体，并正在通过农杆菌浸染法将该基因反义转化至蔷薇科植物中。
In this dissertation, a partial fragment of NAD-SDH from apple fruit was cloned, the NAD-SDH antisense expression plasmid was constructed, and the antisense expression has been doing;
本研究克隆了苹果NAD-SDH 基因的部分cDNA 序列，构建了其反义表达载体，并正在通过农杆菌浸染法将该基因反义转化至蔷薇科植物中。
In this dissertation, a partial fragment of NAD-SDH from apple fruit was cloned, the NAD-SDH antisense expression plasmid was constructed, and the antisense expression has been doing;
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