这是关于美奇酵母属酸性蛋白酶基因克隆和表达的首次研究报导。
All these evidences showed that SAP6 gene cloned in this study indeed encoded the acid protease.
人血管生成素-1基因的克隆及表达载体的构建，为进一步研究其功能、活性和应用奠定了基础。
The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.
表观遗传修饰在基因表达和克隆胚的早期发育方面有重要作用。
Epigenetic modifications plays an important role in gene expression and the early development of cloned embryo.
建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
为研究该酶，克隆和表达该基因片段。
To study N-acetylglucosaminidase, clone and express the gene.
对花粉发育及其特异基因的克隆、表达和调控进行了综述。
The cloning, expression and controlling of specific genes in developing pollen were reviewed briefly.
目的克隆死亡因子相关基因15（MRG15）并研究其在正常晶状体和年龄相关性白内障晶状体上皮细胞中的表达，比较二者的差异。
ObjectiveTo clone the gene of mortality factor related gene 15(MRG15) and to investigate its expression in lens epithelium cell(LEC) of normal and age related cataractous eyes.
要阐明各种信号传递途径与基因表达调控的机理，克隆和鉴定转录因子是关键。
One of the keys to understand the molecular mechanism of gene expression and the pathway of signal transduction is to clone and identify the transcription factors.
目的 对弓形虫表面抗原SAG4基因进行克隆、表达和免疫反应性分析。
To clone and express surface antigen SAG4 gene of Toxoplasma gondii, and analyze its immunoreactivity.
作者介绍了褪黑激素受体1b的结构和功能，褪黑激素受体1b基因的克隆及基因结构、发育性表达与作用、定位与多态性，并讨论了该基因与繁殖季节性的关系。
This review introduced the structure and function of melatonin receptor 1b, the cloning and structure, developmental expression, mapping and polymorphism of melatonin receptor 1b gene.
方法：将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中，研究表达水平的差异。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
文章就高等植物NADP - ME的克隆、功能和基因表达调控方面作一介绍。
This paper is an introduction of the higher plant NADP-ME in the aspect of cloning, function and regulation of gene expression.
目的克隆和表达日本血吸虫钙磷蛋白基因，纯化表达产物。
Objective To clone and express Schistosoma japonicum (Sj) calcyphosine gene, and purify the expressed protein.
目的克隆人、黑猩猩和叶猴FKN全基因及体外表达，比较研究FKN在进化过程中基因组水平和蛋白表达水平的差异。
Objective:To clone full-length FKN gene of human, chimpanzee and leaf monkey and compare their difference in genome and protein expression level.
目的克隆人表皮生长因子受体（HER2）基因，建立共表达HER2基因和雌激素受体（ER）基因的MCF-7细胞模型。
Objective To clone human epidermal growth factor receptor (HER2) gene and establish a MCF-7 cell line with stable co-expression of HER2 and estrogen receptor (ER).
目的：克隆人MAGE3基因并进行原核表达和分离纯化。
AIM: to clone human MAGE 3 gene, to induce its prokaryotic expression and to purify the protein.
本文报道了在克隆了小鼠酪氨酸酶基因的基础上，在大肠杆菌表达体系中诱导表达野生型和变异型酪氨酸酶的结果。
Here we reported the cloning of the gene from murine tyrosinase, the expression of wild tyrosinase and variants in E. coli.
随着生物信息学和基因定位的迅猛发展，EST已成为基因定位、基因克隆、基因表达分析的有力工具。
Along with the development of bioinformatics and genetic localization, EST has already become a powerful tool for mapping, cloning and expression profiling of genes.
该领域国外研究热点在嗜水气单胞菌毒素的基因组结构、相关毒力因子基因克隆和序列比较以及毒素蛋白的表达和致病机理等方面。
In this study, the Aer toxin gene was cloned and expressed in prokaryotic system and the antigenicity of its recombinant protein was analyzed.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
该启动子的成功克隆和其报告基因载体的构建，为研究SOD2的基因表达调控机制提供了重要基础和工具。
The reporter gene vector driven by SOD2 promoter will provide an experimental tool for the further study on the regulatory mechanism of the SOD2 expression.
对该基因的克隆和表达分析为今后研究家蚕胚胎发育及细胞周期调控奠定了基础。
The cloning and expression analysis of BmCcnl1 provide the basis for the research of the embryonic development and cell cycle regulation.
花器官高表达转录因子的克隆将为研究t CP基因调控植物花发育和花形态建成提供新的物质基础。
The cloning of the new member of TCP transcription factor family will provide new base of material for the research of plant flower development regulation and flower tissue forming.
目的亚克隆和表达日本血吸虫钙结合蛋白SjE16的编码基因，初步研究其免疫反应性。
Objective To sub-clone and express the gene encoding Schistosoma japonicum calcium-binding protein (SJE16) and study its immunological response.
本发明提供一种CD86人源化单克隆抗体，其相关基因序列和表达系统。
The invention provides a CD86 humanized monoclonal antibody, an associated gene order and an expression system thereof.
利用序列分析技术克隆与抗逆相关的基因，并通过原核表达体系和转基因技术进行功能验证。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
要阐明各种信号传递途径与基因表达调控的机理，克隆和鉴定转录因子是关键。
Yeast one hybrid system is a new method for isolation and identification of cDNA clones encoding specific DNA binding proteins. Here the procedure of screening DREB transcription factor by ye…
要阐明各种信号传递途径与基因表达调控的机理，克隆和鉴定转录因子是关键。
Yeast one hybrid system is a new method for isolation and identification of cDNA clones encoding specific DNA binding proteins. Here the procedure of screening DREB transcription factor by ye…
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