目的探讨地塞米松对体外培养的骨髓基质细胞增殖和分化等生物学特性的影响。
OBJECTIVE: To investigate the effects of dexamethasone on the proliferation and differentiation of bone marrow stromal cells.
目的:探讨骨髓基质细胞条件培养液诱导中脑神经干细胞分化为高比例神经元的机制。
Objective: To investigate the effects of bone marrow stromal cell conditioned medium on differentiation of mesencephalic neural stem cells into neurons.
目的以原代培养小鼠骨髓基质细胞为饲养层,探讨精原干细胞能否在该饲养层上生长及生长的影响因素。
To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.
目的:研究兔活性骨组织在共培养条件下对同种骨髓基质细胞来源成骨细胞黏附特性的影响。
AIM: To investigate the effects of rabbit living bone tissue by co-culture on adhesion characteristics of homogeneous osteoblasts from bone marrow stromal cell.
结果原代的小鼠培养骨髓基质细胞能很好的维持精原干细胞的非分化性扩增。
The feeder layers of bone marrow stromal cells allowed spermatogonial stem cells to proliferate well and remain undifferentiated.
结论:体外培养的大鼠骨髓基质细胞移植在大鼠骨髓内能够至少存活12周,并在脊髓损伤处发挥一定的修复功能。
CONCLUSION: the cultured bone marrow stomal cells in vitro can survive for 12 weeks at least in the spinal cord of rats, and repair the injured spinal cord to some extent.
目的:应用纤维蛋白胶作为细胞支架进行兔骨髓基质细胞立体培养,探讨其作为新型骨组织工程支架材料的可行性。
AIM: to observe three-dimensional culture of rabbit marrow stromal cells (MSCs) in fibrin glue in vitro, and discuss the feasibility of the fibrin glue to be use as a kind of scaffold material.
取第3代骨髓基质细胞,以含胎牛血清的DMEM培养液培养消化后,计数细胞量,描绘细胞增殖曲线。
After 3 passages were obtained, and then digested by DMEM medium containing fetal calf serum, cell amount was counted and the proliferation curve of MSCs was depicted.
实验部分通过兔疫介导的再障小鼠骨髓基质细胞体外培养探索活髓片在骨髓造血微环境方面的作用机理。
In laboratory research, we observed effect of Huo Shui TAB on the bone marrow micro-environment of immune-mediated aplastic anemia on mice by in vitro culture of bone marrow stromal cell.
应用体外骨髓基质细胞贴壁培养的方法,观察了小鼠骨髓贴壁细胞的形态及其对造血的调控作用。
By using the techniques of in vitro bone marrow long-term liquid culture system, we observed the morphological features of murine bone marrow stroma cells and their hemotopo etic regulation.
目前已建立了体外培养骨髓基质干细胞的方法,而且正在摸索进一步纯化的方法和诱导分化的条件。
Cultivation, purification, as well as induction methods have been developed and that will definitely lead to further clinical application of the cells.
前言:目的:研究体外培养的兔骨髓基质干细胞的成骨诱导及复合培养生长状况。
AIM: To study the induction of osteoblasts by rabbit bone marrow stromal stem cells (BMSSCs) and the growth status of the cells after co culture.
目的: 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞,优化培养条件,建立饲养细胞系,为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
方法将骨髓基质细胞与牙骨质片和股骨片在体外共同培养,分别在第7天、第28天进行光镜观察及碱性磷酸酶的测定。
Methods BMSCs were co-cultured with cementum slices and femora slices in vitro, to detect with light microscope at 7 day and 28 day, and to determine ALP activity.
骨髓基质干细胞是一种由骨髓中分离获得的具有多种分化潜能的间质干细胞。在体外培养条件下,它可以分化为成骨细胞、软骨细胞、脂肪细胞、甚至于成肌细胞。
Bone marrow stromal stem cells isolated from bone marrow are the special mesenchymal stem cells, which can be differentiated in culture into osteoblasts, chondrocytes, adipocytes, and even myoblasts.
在中药治疗骨质疏松症的药物筛选,骨髓基质细胞培养与组织工程人工骨研究。
In Chinese medicine the treatment of osteoporosis drug screening, bone marrow stromal cell culture and tissue engineering artificial bone research.
在中药治疗骨质疏松症的药物筛选,骨髓基质细胞培养与组织工程人工骨研究。
In Chinese medicine the treatment of osteoporosis drug screening, bone marrow stromal cell culture and tissue engineering artificial bone research.
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