本文分析了从大麦成熟胚培养快速建立胚性悬浮细胞系的两个关键步骤。
The present paper analysed two key procedures for rapid establishment of embryogenic cell suspension cultures derived from barley mature embryo.
目的体外建立可长期培养的抗原特异性T细胞系及克隆，并分析其T细胞受体的使用情况。
Objective To establish long-term cultured antigen-specific T cell line and clones and to analyze their T cell receptor usage.
并采用酶联免疫吸附试验，用原代培养的正常胶质细胞、胶质瘤细胞以及胶质瘤细胞系对这52例胶质瘤患者进行检测和筛选。
The 52 glioma patients were detected and filtered with primary cultured normal glial cells, glioma cells and glioma cell line by enzyme linked immunosorbent assay.
优化了悬浮细胞系培养的条件：悬浮细胞系的继代时间、谷氨酰胺对悬浮细胞系的生长产生影响。
Culture condition of cell suspension was optimized, the growth of suspension cell was affected by Gin content added and interval of subculture.
应用体外细胞培养和MTT比色法。研究大豆异黄酮类化合物金雀黄素和大豆黄酮对人乳腺癌细胞系体外增殖作用的影响。
The effects of genistein and daidzein on the proliferation of human breast cancer cell line in vitro were studied by cell culture and MTT colorimetric method.
变异细胞系经无盐继代培养后仍具有较高的游离脯氨酸积累能力，表明变异细胞系具有稳定的耐盐性。
The variant cell lines still have high ability of accumulating free proline after cultivation on normal medium, which indicated the variant cell lines have steady salt tolerance.
方法用MTT检恻LHRH-PE40对体外培养的人肿瘤细胞的细胞毒作用，找出敏感肿瘤细胞系。
Methods The cytotoxic activity of LHRH-PE40 to the human tumor cell lines cultured in vitro was detected by MTT method, and sensitive tumor cell lines were selected out.
因为我们可以把分离这些细胞并且进行体外培养，我们就可能培育‘健康’和‘不受影响’的细胞系。
Because we can separate the cells and culture them individually, we could potentially generate 'healthy' and 'affected' cell lines.
方法建立RPE与抗原特异性T淋巴细胞系和静止淋巴细胞之间的共培养系统。
Methods Co culture systems of RPE cells with antigen specific t lymphocyte lines and resting t lymphocytes were established in vitro.
利用高效液相方法观察了氯化钾对培养人神经母细胞瘤细胞系(SHSY5Y)细胞谷氨酸释放的影响。
The influence of KCl on the release of glutamic acid from culture human neuroblastoma cell line (SH SY5Y) was observed by using HPLC.
众多不同的细胞诸如猪肝、人肝细胞或细胞系均已应用，也研究了不同的培养方法，以期完善生物组分的活性。
Many different cells including porcine, human hepatocytes or cell lines have been used and different culture methods have been studied to optimize the activity of the biological component.
目的了解乳腺癌细胞系mcf - 7中是否存在乳腺癌干细胞，以及在不同的培养条件下，干细胞池的变化情况。
Objective To know if MCF-7 breast cancer cell line has breast cancer stem cell, and change of stem cell pool in diverse incubation circumstance.
目的探索人眼视神经星形胶质细胞体外培养方法，建立细胞系，为进一步研究视神经损伤后的修复提供充足的细胞来源。
Objective To culture astrocytes of human optic nerve and establish the cell lines for further study of healing process after optic nerve trauma.
选用新生SD大鼠的髁状突软骨作为原代细胞培养的组织来源，建立髁状突软骨细胞有限细胞系；
The primary MCC separated from the mandibular condylar cartilage of six neonatal SD rats were cultured and a finite cell line was established in vitro.
结果建成细胞系CM 319，经近两年的体外培养，已连续传代百余次。
Results The newly established cell line CM 319 has been maintained in continual cultures for over 100 generations in two years.
目的建立体外培养的正常人表皮黑素细胞系，检测壬二酸对人表皮黑素细胞和鼠黑素瘤B16细胞黑素合成的影响。
Objective To establish human normal melanocytes cell line in vitro and observe the effects of Azelaic acid on the pigment production of the epidermal melanocytes and B16 cells.
目的：采用原代培养的方法建立一株人肝癌细胞系ehh1，并对其生物学特性进行分析。
Objective: to establish a human hepatocellular carcinoma cell line EHH1 by primary culture and to investigate its biological characteristics.
为了更为有效地利用该模型小鼠，我们通过原代培养建立了该模型的肿瘤细胞系。
We also established the cell line of this transgenic mice model through primary cell culture to make use this model more efficiently.
方法：采用细胞培养的方法建立稳定传代的胰腺癌hs 766t细胞系，体外培养人脐带静脉内皮细胞。
Methods: We built the pancreatic cancer cell group HS 766t and human endothelium cell in the umbilical vein by the way of cell culture in vitro.
悬浮培养细胞系是植物生理生化研究的好材料之一。
Cell suspensions are valuable materials for plant physiology and biochemistry studies.
精原干细胞的成功培养为今后重建完整的生精细胞系的治疗性移植和对这类定向干细胞的发育及分化潜能的研究提供了细胞模型。
The cultivation of SSCs may provide an ideal cell model for the research of committed stem cells' development and differentiation, which can be applied in remedial transplantation.
实验目的研究体外培养GCT细胞的生物学特性，建立一株人gct细胞系。
AIM: To study the biological characteristics of GCT of bone cultured in vitro and establish a human cell line from GCT of bone.
在体外培养条件下获取纯度较高的少突胶质细胞系细胞并探索其分化规律。
The present study is to obtain more pure oligodendrocytes and further understand the committed differentiation of oligodendrocyte lineage cell in vitro.
详细系统地说明了迄今为止建立人胚胎干细胞系所需要的饲养层类型、培养基组成、添加细胞因子种类及其作用；
Selection of the feeder layer, medium, and supplemental cytokines and their roles in establishing and maintaining human ES and EG cell lines at present are illustrated in detail systematically.
方法：利用体外细胞培养、MTT、NBT染色及吞噬功能测定等方法，观察在不同浓度金龙胶囊作用下人早幼粒白血病细胞系(HL - 60)的细胞形态和功能的变化。
Methods: in vitro cell culture, MTT titration, NBT reduction and phagocytosis determination were employed to study the effects of JLC on HL-60 cells.
目的：培养建立中国人种前列腺癌细胞系，为了解中国人种前列腺癌细胞生长特性、进一步开展前列腺癌基因诊断和药物筛选建立方便的实验手段和模型打下基础。
Objective: To culture and establish Chinese ethnic prostate cancer cell line, investigate its growth characterization and explore experimental methods and models of gene diagnosis and trial of drugs.
本发明无血清虹鳟胚胎细胞系培 养基培养虹鳟胚胎细胞系具有增殖速度快，传代时间长的优点。
The rainbow trout embryo cell line medium cultured by the serum-free rainbow trout embryo cell line medium of the invention has the advantages of rapid breeding speed and long regenerating cycle.
本发明无血清虹鳟胚胎细胞系培 养基培养虹鳟胚胎细胞系具有增殖速度快，传代时间长的优点。
The rainbow trout embryo cell line medium cultured by the serum-free rainbow trout embryo cell line medium of the invention has the advantages of rapid breeding speed and long regenerating cycle.
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