结果人羊膜上皮细胞可以在体外成功的培养传代，体外可连续传8 ~10代。
Results Human amniotic epithelial cells were successfully cultured and passaged in vitro, and could be passaged successively for 8-10 times.
作者引进并建立了大鼠小肠上皮细胞(IEC—6)的培养传代和活性检测的实验方法；且对几种影响因素进行了探讨。
A culture method and assay for the rat small intestinal epithelial cell (IEC-6) line have been established and factors that influence the results of the culture have been investigated.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
结论：利用多次差速传代可从混合培养的原代细胞中获得纯化的牙囊细胞。
Conclusion: Dental follicle cells can be purified by several differential passages from the mixed primarily cultured cells.
结果流式细胞术证明，MSC具有间充质细胞的特征。原代及传代培养显示，胎儿骨髓msc具有活跃增殖的能力。
Results Flow cytometry analysis showed that fetal bone marrow derived MSC exhibited the characteristics of mesenchymal cells, MSC had an active proliferative ability in primary and passage cultures.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
培养的细胞可以稳定生长传代。
本文就胚胎质量和胚胎类型分化抑制物培养基传代时间和消化液等方面对胚胎干细胞分离克隆的影响进行了讨论。
Influences of embryo quality and type differentiation stayer media passage and digestion fluid on embryonic cell separation and cloning were discussed respectively.
方法将人肝门部胆管癌标本进行细胞培养，传代。
Methods The fresh human hilar cholangiocarcinoma specimen was cultured.
本文综述该病原体国内外报道的分离培养方法，主要包括样品处理、培养条件、虫株传代、虫株致病性检测和虫株保存方法。
This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passage culture, pathogen detection, and maintenance.
另外研究血管内皮生长因子(VEGF)和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。
In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis.
方法分别自3周龄大耳白兔关节软骨和半月板分离软骨细胞，行单层传代培养和离心管培养。
Methods Chondrocytes isolated from articular cartilage and meniscus of rabbits aged 3 weeks were respectively passaged in monolayer and cultured in centrifuge tube.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
方法：原位传代培养羊水细胞并制备染色体，G显带分析核型，产后随访。
Methods:Amniotic fluid cells were cultured in situ and their karyotypes were analyzed by G band, followed them after delivery.
结果原代培养的细胞ALP染色阳性区域可以达90%以上，传代培养后细胞阳性率为100%。
Results the area positive for ALP staining is no less than 90% in the primary culture and almost 100% in the subculture.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
方法：取新西兰白色家兔晶体上皮细胞进行原代培养，细胞融合后用胰蛋白酶进行消化传代。
Methods New Zealand white rabbit lens epithelial cells were primarily Cultured. After the confluence, the cells were trysinized and sub-cultured.
猪的复合皮肤替代物的培养情况：培养第3天在真皮基质上加入传代后的猪角质形成细胞，其上可见薄的角质形成细胞层。
Culture of pig composite dermal substitute pig keratinocytes after passage were added on the dermal matrix on the 3rd day of the culture and thin pig keratinocyte layer was found.
使用标准培养液的传代细胞均为阴性。
The cells, which were cultured in standard culture medium, were negative.
自成年新西兰兔股骨转子部取得骨髓基质细胞进行传代培养，所得骨髓基质细胞再与钙磷陶瓷载体复合培养制成复合人工骨。
Bone marrow cells from New Zealand white rabbits were cultured to obtain stromal cells, which were then cultured with the carriers to make the artificial composite bone graft.
结论人羊膜上皮细胞在体外可成功进行原代和传代培养，体外培养的人羊膜上皮细胞在一定时间内可维持增殖能力。
Conclusion Human amniotic epithelial cells can be successfully primary cultured and passaged in vitro, and the cells can proliferate for a certain period.
较离心法简便的聚已二醇法可用于沙眼衣原体的培养及传代；
The PEG handling is better than the centrifugation at the separated and culture passage of Ct.
方法利用手术显微镜及显微手术器械分离大鼠肠系膜淋巴管。采用植块培养法进行内皮细胞原代培养并传代培养。
Methods With an operating microscope and microsurgical instruments, rat's mesenteric lymphatics were isolated and the endothelium were primarily cultured and subcultured.
采用无血清液体培养基、DMEM培养基和MEM培养基进行PK15细胞的培养，观察细胞增殖及传代效果，确定无血清液体培养基的营养功能。
PK15 cells were cultured with free-blood serum media, DMEM media and MEM media in other to observe the effect of cell proliferation, cell transfer and define the ability of nutrition.
方法：用胰酶消化法培养兔rpe细胞并传代。
Methods: The rabbit RPE cells were cultured with trypsin enzyme-digesting technique and passaged in vitro.
经6次传代培养后，其生物量和菌丝多糖产量较稳定，且明显超过原始菌株，表明其遗传稳定性良好。
After continuous cultivation for 6 generations, the biology yield and the polysaccharide yield were steady and obviously exceeded the original strain. It showed a good genetic stability.
方法从人胚胎海马区分离神经干细胞，采用无血清培养基，进行体外扩增培养、传代。
Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus.
方法用狗肾传代MDCK细胞培养法观察儿茶提取物抑制甲型流感病毒的致细胞病变作用；
Methods The inhibitory cytopathic effects (CPE) of catechu extract on influenza A virus were observed by MDCK cell culture techniques.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
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