方法体外培养人脐静脉内皮细胞，通过传代形成细胞的衰老模型。

Methods Human umbilical vein endothelial cells (HUVECs) were cultured in vitro.

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前言：目的研究养阴方对培养人脐静脉内皮细胞内皮素(et)和一氧化氮(NO)含量的影响。

Objective: to Study the influence of Yangyin Recipe to the concentration of Endotheliolyisin (ET) and Nitrogenmonoxide (no) by cultivating endotheliocyte of human umbilical vein.

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结论葛根素可以缓解培养人脐静脉内皮细胞内钙超载，其作用机制可能与拮抗膜上电压依赖型钙通道和抑制内质网钙库释放有关。

Conclusion the mechanism of puerarin reducing the overload of calcium in HUVECs may be related with blocking both voltage-dependent calcium channels and the release of calcium pool in endoplasm.

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结论葛根素可以缓解培养人脐静脉内皮细胞内钙超载，其作用机制可能与拮抗膜上电压依赖型钙通道和抑制内质网钙库释放有关。

Conclusion the mechanism of puerarin reducing the overload of calcium in HUVECs may be related with blocking both voltage-dependent calcium channels and the release of calcium pool in endoplasm.

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