目的研究浙江省手足口病患者中肠道病毒（EV）71型分离株的病毒基因特征。
Objective To study the gene characterization of enterovirus 71(EV71) virus strains isolated from clinical specimens of children with hand-foot-and-mouth disease (HFMD) in Zhejiang province.
目的制备登革2型病毒中国分离株(DEN2 - 43)的衣壳蛋白缺失突变病毒。
Objective To generate rescued viruses with deletion mutation of capsid protein from dengue virus type 2 isolated in China (DEN2-43).
目的研究柯萨奇B组3型病毒（CVB3）中国分离株结构蛋白VP4、非结构蛋白3D基因序列及变异性。
Objective To investigate the sequence and variation of VP4 and 3D gene of a coxsackievirus B3 Chinese isolate(CVB3-Chi).
目的分离培养人疱疹病毒6型(HHV - 6)青岛地方株并进行初步鉴定。
Objective To isolate and identify human herpesvirus 6 (HHV-6) local strains of Qingdao.
对我国部分省市粮食粉尘中的苏芸金芽孢杆菌（B t ）野生株的进行分离、纯化及H血清型鉴定。
To isolate the Bacillus thuringiensis native strains from warehouses grain dusts in China and analyze their H antigen serotypes.
目的旨在分离人类疱疹病毒7型（HHV 7）的南京地方株。
Objective The present research intends to isolate Nanjing local strains of human herpesvirus 7 (HHV 7).
对从郎酒厂高温大曲中分离出的14株高温型红曲霉的培养温度和保藏条件进行了初步研究。
The culture temperature and storage conditions of 14 high-temperature monascus strains separated from high-temperature Daqu in Langjiu Distillery were studied.
从大棚土壤中分离到一株异养型硝化细菌，命名为菌株HN ，分离菌株为革兰氏染色阳性，球状或杆状。
A heterotrophic nitrifier, named strain HN, was isolated from the greenhouse soil. The cells of isolates were Gram positive, rod or coccus.
结果发现47株马拉色菌临床分离株可分为6型。其DNA型别与孢子形态之间无绝然对应关系。
The results showed that 47 clinical isolates of Malassezia species could be classified into 6 types based on their DNA banding patterns.
用鸡肾型传支病毒（IBV）分离株的鸡胚尿囊液接种16日龄蛋雏鸡，并随机分为对照组、中药治疗组和卵抗治疗组。
Allantoic fluid of chicken embryo that had been inoculated with one strain of renal type IBV was used to challenge 16-day-old chicken.
目的对不同来源的12株肠炎沙门菌分离株进行分子分型。
Objective Molecular typing 12 strains of the Salmonella enteritidis isolated from different origins.
目的研究不同口腔黏膜病白念珠菌分离株的基因型特点。
Objective To assess the genotypic characteristics of oral Candida albicans isolates from different oral mucosal disease patients.
方法对临床分离的15株金黄色葡萄球菌，酚氯仿法提取其DNA后行RAPD分型，同时进行噬菌体分型。
METHODS DNA from 15 clinical strains of S. aureus were extracted by phenol-chloroform method and used in the RAPD assay, phage typing were also performed.
方法对临床分离的36株肺炎克雷伯菌进行RAPD基因分型，并通过指纹图谱比较与分析，确证医院感染爆发。
Method RAPD was used to genotype the 36 strains of Klebsiella pneumoniae isolated from the clinical samples and compare the fingerprint to confirm hospital infection outbreak of Klebsiella pneumoniae.
型魏氏梭状芽孢杆菌XP5株分离自典型猝死症病猪，将其制成氢氧化铝灭活疫苗。
An inactivated aluminum hydroxide adjuvant bacterin was produced with the Clostridiun welchii type A strain XP5 which was isolated from a typical sudden death diseased pig.
直接PCR法对新疆牛结核分枝杆菌分离株的分型结果显示出，PCR指印带型中带数较少，且与北京牛结核分枝杆菌分离株的PCR指印带型截然不同。
The PCR fingerprints of Xinjiang strains were quite different with Beijing strains', PCR fingerprints of Xinjiang strains had a fewer bands than Beijing strains.
我们建立了1种易于使用的于含15种染色体微卫星标志的多重PCR中对鼠弓形虫进行分离株基因分型的方法。
We developed an easy-to-use method for genotyping Toxoplasma gondii isolates in a single multiplex PCR assay with 15 microsatellite markers.
从表现类似新城疫症状的病死半番鸭中分离到1株病毒FM01株，经血凝及血凝抑制试验证实为禽1型副粘病毒。
A strain of virus named FM01 was isolated from the liver of dead semi-muscovy ducks characterized similar to Newcastle disease. It was determined to be a paramyxovirus type 1 by HA and HI tests.
方法：对临床分离的85株金黄色葡萄球菌，用酚氯仿法提取DNA后进行RAPD分型。
Methods: 85 clinical strains of S. aureus were extracted by phenol chloroform method and the RAPD typing was also performed.
结论甲型H1N1分离株的变异特征及亲缘进化，分析对流感的流行病学研究具有重要意义。
Conclusion the variation and phylogenetic analysis of the influenza a (H1N1) virus are of great importance to the epidemiological study of influenza.
在33株从腹泻病患者粪便标本分离的包括6种血清型的福氏志贺氏菌株中有 30株携带该基因岛。
The new genomic island was detected in 30 of 33 strains isolated from fecal samples of patients with diarrhea, including infections with 6 serotypes of Shigella flexneri.
结合病毒分离鉴定确诊为PRRS病毒（PRRSV）感染，RTPCR试验证实分离毒属美洲型PRRSV ，且与ATCCVR2 332株属同一基因亚型。
RT PCR test proved that the isolate belongs to serotype of American PRRSV and shares the same subgenotype with ATCC VR2332.
结果发现47株马拉色菌临床分离株可分为六型。其DNA型别与孢子形态之间无绝然对应关系。
The results showed that 47 clinical isolates of Malassezia spp. could be classified into 6 types based on their DNA banding patterns.
目的用PCR方法扩增红色毛癣菌和须癣毛癣菌临床分离株的DNA ，观察PCR指纹的差异以及基因型与传统表型的关系，建立一种红色毛癣菌分子生物学鉴定方法。
Objective To observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum , and establish an effective molecular method to identify T. rubrum .
目的用PCR方法扩增红色毛癣菌和须癣毛癣菌临床分离株的DNA ，观察PCR指纹的差异以及基因型与传统表型的关系，建立一种红色毛癣菌分子生物学鉴定方法。
Objective To observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum , and establish an effective molecular method to identify T. rubrum .
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