结论:合适的福尔·马林固定石蜡包埋组织dna提取方法可应用PCR进行DNA水平的相应的研究。
Conclusion: Appropriate DNA extract methods for formalin fixed and paraffin embedded tissues may benifit PCR.
由于甲醛介导的DNA损伤和石蜡对DNA提取的阻碍作用,使得常规DNA提取方法很难从福尔马林固定石蜡包埋组织(FFPET)中获取高质量的DNA。
Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue(FFPET).
全部组织均经10%福尔马林固定,石蜡包埋,术前均未行放疗,化疗或者其他生物治疗。
The tissues were fixed in 10% formalin and embedded in paraffin. No patients had received preoperative chemotherapy , radiation therapy or other biological therapy.
搜集裸鼠各脏器组织,10%缓冲福尔·马林固定,常规石蜡包埋,HE染色,镜下观察形态学改变。
Gathered viscus tissues of nude mouse, fixed with 10% dampen formaldehyde solution, paraffin embed, routine he stained and morphology observed by microscope.
目的探讨固定剂及固定时间对石蜡包埋rna病毒感染组织中RNA降解及标本病理形态学的影响。
Objective To study the effect of fixatives and fixed_time on the morphology and detectability of viral RNA from paraffine_embedded tissues by RT_PCR.
方法福尔·马林固定、石蜡包埋40例肉瘤标本进行HE和TSG101免疫组织化学染色,观察tsg101的表达情况。
Methods 40 formalin fixed and paraffin embeded sarcoma tissues were stained by he and immunohistochemistry to observe the expression of TSG101.
并取肝左叶于10%中性甲醛溶液中固定,石蜡包埋,作组织形态学观察。
Left lobe of liver was fixed in 10% formaldehyde, than embedelinged in paraffin, at last observed histological change with optical microscope.
大脑组织形态学观察:大鼠脑组织用甲醛固定24小时,石蜡包埋,制作病理切片,HE染色。
Observation of brain tissues morphology: Rats brain tissue with formaldehyde 24 hours, embedded in paraffin, making pathological section, HE stain.
从患可手术的乳腺癌患者中获得93例福尔·马林固定石蜡包埋(FFPE)的组织,来验证RASSF1A甲基化的预后重要性。
The prognostic significance of RASSF1A methylation was validated in 93 formalin fixed paraffin-embedded (FFPE) tissues obtained from patients with operable breast cancer.
搜集裸鼠各脏器组织,0%缓冲福尔·马林固定,常规石蜡包埋,HE染色,镜下观察形态学改变。
Gathered viscus tissues of nude mouse, fixed with 0% dampen formaldehyde solution, paraffin embed, routine he stained and morphology observed by microscope.
搜集裸鼠各脏器组织,0%缓冲福尔·马林固定,常规石蜡包埋,HE染色,镜下观察形态学改变。
Gathered viscus tissues of nude mouse, fixed with 0% dampen formaldehyde solution, paraffin embed, routine he stained and morphology observed by microscope.
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