采用四氮甲基唑蓝比色法、溴乙锭荧光法检测肺细胞的生长情况和DNA交联的形成。
The cytotoxicity and DNA cross links of the cells were detected with MTT assay and ethidium bromide fluorescence assay.
方法:四氮唑蓝比色法(MTT法)观察梁金菇多糖对HL 6 0细胞的抑制率,应用形态学、流式细胞术、DNA凝胶电泳等方法检测细胞凋亡的发生。
METHODS: The proliferation inhibition effects of LJPS on HL-60 cells was observed by MTT colorimetry. Apoptosis was detected by morphology, flow cytometry and DNA agarose gel electrophoresis.
方法:四氮唑蓝比色法(MTT法)观察梁金菇多糖对HL 6 0细胞的抑制率,应用形态学、流式细胞术、DNA凝胶电泳等方法检测细胞凋亡的发生。
METHODS: The proliferation inhibition effects of LJPS on HL-60 cells was observed by MTT colorimetry. Apoptosis was detected by morphology, flow cytometry and DNA agarose gel electrophoresis.
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