免疫荧光法检测神经元标志神经特异性烯醇化酶(nse)和神经丝蛋白(NF)的表达。
The expressions of neuronal markers NSE (neural specific enolase), NF (neurofilament), and glial cell marker GFAP (glial fibrillary acidic protein) were detected by fluorescent immunohistochemistry.
方法采用酶联免疫吸附法对40例特发性肺纤维化患者和30例健康人血清基质金属蛋白酶- 9进行测定。
Methods The serum level of MMP-9 in 40 patients with IPF and 30 healthy subjects was measured by ELISA.
方法：分别用葡萄糖氧化酶法和放射免疫法对35例危重病儿和17例轻症病儿血糖和胰岛素进行测定。
Method:The levels of blood glucose, insuline were observed in 35 children with critical disease and 17 children with upper respiratory infection or bronchits.
方法将123例患者的血清分别用化学发光法和酶联免疫法检测，并做重复性实验。
Methods the serum of 123 cases of patients were detected with ECLIA and ELISA respectively, and the repeatability tests were carried on, then.
目的：通过对酶联免疫吸咐试验(ELISA)双抗原夹心一步法和二步法检测人类免疫缺陷病毒(HIV)抗体的比较，探讨各自特点。
Objective: To compare the characteristics of the one step and two steps ELISA (Enzyme Linked Immunosorbent Assay) diagnostic kit in detecting of human immunodeficiency virus (HIV) antibodies.
方法采用酶联免疫法检测糖皮质激素不同给药方式治疗前后外周血清及诱导痰液中IL-4、IL-5和ECP水平。
Methods The levels of IL-4, IL-5 and ECP in the serum and induced sputum were measured with enzyme-linked immunosorbent assay (ELISA) method before and after two different treatments.
方法：采用放射免疫分析法和硝酸还原酶法测定20例HIE新生儿和20例正常新生儿血浆et与血清NO水平。
Methods: Plasma et levels were determined by using a radioimmunoassay kit, serum no levels were determined by using a nitrate reductase method in 20 newborn with HIE and 20 normal newborn.
目的比较酶联免疫（ELISA）、放射免疫（RIA）和免疫印迹（IB）法检测胰岛素自身抗体（IAA）敏感性和特异性的差别。
Objective To compare the difference of enzyme-linked immunosorbent assay(ELISA), Radioimmunoassay(RIA) and immunoblotting(IB) in the determination of insulin autoantibody(IAA).
目的：了解磁珠酶联免疫分析法检测人类白细胞抗原-B27的准确性、敏感性和特异性，探讨其在强直性脊柱炎辅助诊断中的价值。
OBJECTIVE: To explore the accuracy, sensitivity and specificity of IMS-ELISA for detecting HLA-B27 and its value in the auxiliary diagnosis of ankylosing spondylitis.
方法免疫组化s - P法检测基质金属蛋白酶MMP - 2在成釉细胞瘤中的表达和分布。
Methods to detect the expression and site of matrix metalloproteinase-2 (MMP-2) in ameloblastoma suing S-P immunohistochemistry.
目的建立使用间接酶联免疫吸附法(ELISA)检测人血浆抗碳酸酐酶iv抗体的方法，阐明血浆抗碳酸酐酶iv抗体滴度与肾病综征合(ns)、慢性肾小球肾炎(CGN)和2 -型糖尿病肾病(2 - DN)三种肾脏病的联系。
Objective to develop a indirect ELISA method for the detection of plasma anti-CA iv antibody and to evaluate the relationship between anti-CA iv antibody with pathogenesis of ns, CGN and 2-dn.
应用亲和酶标组化标记法和免疫组化ABC法，测定106例乳腺癌组织中ER、PR和DAKO—M1水平。
The expression of ER, PR and DAKO-M1 in 106 breast carcinomas were examined by bothaffinitive histochemical and ABC technique.
用SDS -聚丙烯酰胺凝胶电泳、酶联免疫法和高效液相测纯度。
At last it was measured by SDS-PAGE, Enzyme-linked immunosorbent assay and HPLC.
分别应用放射免疫法和酶动力法测定血清透明质酸（HA）和淀粉酶。
Radioimmunoassay was performed to determine the serum level of hyaluronic acid (HA).
MTT法观察体外gah对肿瘤细胞生长和小鼠淋巴细胞增殖的影响，酶联免疫法观察gah对淋巴细胞分泌细胞因子il - 2的影响。
Methods The effects of GAH on antitumor and lymphocytes proliferation were observed by MTT, and IL-2 were observed by ELISA.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
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