方法：采集健康的牙龈组织和牙周膜，体外培养牙龈成纤维细胞和牙周膜成纤维细胞。
Methods: Healthy human gingiva and periodontal ligament were acquired from patients, then gingival fibroblasts and periodontal cells were cultured in vitro.
目的：体外观察尼古丁和烟草浸提液（ST）对人牙龈成纤维细胞的生长和贴附影响。
Objective:To observe the effects of nicotine and smokeless tobacco extract (ST)on growth and attachment of human gingival fibroblast.
目的：体外观察尼古丁和烟草浸提液（ST）对人牙龈成纤维细胞的生长和贴附影响。
Objective:To observe the effects of nicotine and smokeless tobacco extract (ST)on growth and attachment of human gingival fibroblast.
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