方法:采集健康的牙龈组织和牙周膜,体外培养牙龈成纤维细胞和牙周膜成纤维细胞。
Methods: Healthy human gingiva and periodontal ligament were acquired from patients, then gingival fibroblasts and periodontal cells were cultured in vitro.
结果:补肾中药醇提活性成分对人牙周膜细胞增殖能力和ALP活性有增强作用。
Results: the active components extracted by alcohol strengthened the proliferation ability and ALP activity of cultured human periodontal ligament cells.
目的:观察中间普氏菌和脆弱类杆菌的内毒素对人牙周膜细胞增殖和超微结构的影响。
AIM: to examine the effect of endotoxin of prevotella intermedia and bacteroides fragilis on proliferation and ultrastructure of periodontal ligament cells in vitro.
它还可诱导牙周膜细胞的增殖和分化。
Furthermore, it can also stimulate proliferation and differentiation of periodontal ligament cell.
方法:通过原代和传代培养,获得性状稳定的人牙周膜细胞,使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载。
METHODS: Primary human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain at different time.
方法:通过原代和传代培养,获得性状稳定的人牙周膜细胞,使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载。
METHODS: Primary human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain at different time.
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