方法：应用荧光标记单克隆抗体和流式细胞技术。
METHODS: Using fluorescent-labeled monoclonal antibodies and flow cytometry.
方法：采用流式细胞仪单染色和三色荧光标记技术。
Methods: to use mono color and three color fluorescent staining methods by flow cytometry.
方法采用MTT法和流式细胞仪技术。
流式细胞仪(FCM)是综合了多项现代技术的科学仪器，在生物学、基础医学和临床医学获得了广泛应用。
Flow cytometry (FCM) which colligated a few of modern techniques is widely used in biology, basic medicine and clinical medicine.
用间接免疫荧光染色结合流式细胞技术检测细胞表面DR4和DR5分子表达；
The expression of cell surface-bound DR4 and DR5 were determined by indirect fluorescence staining and flow cytometry analysis.
结果①流式细胞术分选后共聚焦显微镜分析技术（PSC）能同时、准确地鉴别不同细胞周期时相的凋亡细胞和非凋亡细胞。
Results Post-sorting confocal (PSC) method could exactly distinguish apoptotic and non-apoptotic cells in the specific cell cycle phases at the same time.
方法应用流式细胞术和细胞免疫荧光染色技术。
Methods It was studied by flow cytometry and immunofluorescence.
方法：采用细胞免疫荧光染色技术和流式细胞术。
Methods Flow cytometry and immunofluorescence staining technique were used.
用TUNEL技术和流式细胞仪技术测定了COPD患者外周血PMN和AM的凋亡变化及其规律。
The dynamics changes of apoptotic polymorphoneuclear(PMN) and AM in COPD patients were measured by flow cytometry and TUNEL techniques.
方法采用LRP单克隆抗体、流式细胞技术分别测定15例单纯缺铁性贫血患者（对照组）和65例AL患者（AL组）LRP的表达率。
The expression rate of LRP was detected by its monoclonal antibody and flow cytometry in 15 asiderotic anemia patients and 65 AL patients.
方法：采用荧光显微镜、电子显微镜、激光扫描共聚焦显微镜、流式细胞分光光度计和DNA凝胶电泳等方法和技术观察细胞凋亡。
Method: Using fluorescence microscopy, electron microscopy, laser scanning confocal microscopy, flow cytometry and DNA gel electrophoresis to demonstrate the presence of apoptosis.
方法采用流式细胞仪技术对不同培养时间和不同培养条件下脐血cd 34 +细胞中pcna的表达水平和细胞周期进行了测定。
Methods Using flow cytometry, PCNA and cell cycle in CB CD34 + cells were measured in different conditions and different culture time.
采用双标记流式细胞分析技术，分别检测各组小鼠脾脏及肠系膜淋巴结（MLN）CD80M和CD86 M的表达。
The number of CD80/CD86 M of spleen and mesenteric lymph node(MLN) were determined by double-label FCM method.
并用TUNEL技术和流式细胞仪检测了相关淋巴细胞的凋亡。
TUNEL and flow cytometry were used to detect apoptotic cells.
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
如巧克力和谷类食品的酒吧早已包装用流式细胞包装技术。
Foods such as chocolate and cereal bars have long been packaged using flow wrapping technology.
方法分别采用流式细胞技术和散色比浊法测定HSP急性期与恢复期及对照组儿童血清免疫球蛋白浓度和T淋巴细胞亚群水平。
Methods The immunoglobulin concentration and T-cell subgroup level of acute stage and convalescent stage HSP children were detected with FCM and nephelometer.
以荧光试剂标记配体法，利用流式细胞仪技术和激光扫描共聚焦显微镜技术研究姜黄素对人淋巴细胞LDL - R表达的影响。
A laser scanning confocal microscopy (LSCM) and a fluorescence flow cytometric methods (FFCM) were used to identify the effect of curcumin on the expression of LDL-R in the human lymphocytes.
方法：实验分人参皂甙组、对照组和GM -CSF组，采用流式细胞技术测定各组细胞的凋亡率。
Methods: the experimental subjects were divided into GS group, control group and GM-CSF group. FACs technology had been used to determine the apoptosis ratio.
采用硝酸甘油偏头痛模型大鼠，运用透射电镜和流式细胞仪技术进行研究。
Methods To choose disease model of migraine with nitroglycerin by means of flow cytometry and transmission electron microscope.
近年来将流式细胞术和荧光微球结合起来，一种新兴的流式荧光微球技术应运而生。
In recent years, with the combination of flow cytometry and fluorescent microsphere, a newly developing technology known as flow cytometry based fluorescent microsphere emerged.
目的：通过高效液相、流式细胞仪技术和MTT发研究紫杉醇对MCF-7的抑制作用。
Objective: by HPLC, flow cytometric technology and research on MCF MTT hair taxol inhibition - 7.
目的：通过高效液相、流式细胞仪技术和MTT发研究紫杉醇对MCF-7的抑制作用。
Objective: by HPLC, flow cytometric technology and research on MCF MTT hair taxol inhibition - 7.
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