介绍采用双光子激发荧光方法进行单分子探测的原理和自行研制的实验装置，激发光聚焦和荧光收集采用共焦方式。

We report here briefly the principle and the device of our single-molecule detection spectrometer based on two-photon excited fluorescence.

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介绍采用双光子激发荧光方法进行单分子探测的原理和自行研制的实验装置，激发光聚焦和荧光收集采用共焦方式。

We report here briefly the principle and the device of our single-molecule detection spectrometer based on two-photon excited fluorescence.

youdao