方法：应用免疫组织化学和免疫细胞化学技术。
METHODS: Technique of immunohistochemistry and immunocytochemistry were used.
用蛋白质印迹免疫检测技术和免疫细胞化学技术鉴定细胞表型。
Immunocytochemistry and western blotting technology were used to identify the cell phenotype.
方法采用组织压印片技术和免疫细胞化学染色。
Methods Tissue imprint and immunocytochemical staining were made from the neurons of IMG.
用HRP逆行标记技术和免疫细胞化学双重标记法结合切断实验印证了NG内神经元向NTS投射的纤维性质。
HRP retrograde transport and PAP double labelling method combined with nodose ganglionectomy to detect nature of projecting fibers from the NG to the NTS.
应用免疫细胞化学技术研究BDNF、GDNF蛋白在体外培养正常神经干细胞和缺氧损伤神经干细胞中的表达以及脑溢安的影响。
Using immunocytochemistry to study the expression of BDNF and GDNF in normal NSC and NSC that had been damaged by hypoxia.
方法免疫细胞化学和原位杂交技术。
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
结论：利用密度梯度离心和贴壁筛选的方法可以分离hmsc，联合应用免疫细胞化学、细胞化学和电镜等技术手段鉴定HMSC的方法有效。
Conclusion The HMSC can be isolated with density-grad centrifugal and anchoring screening. The method to identify HMSC with immunocytochemistry, cytochemistry and electron microscopy is effective.
结论：利用密度梯度离心和贴壁筛选的方法可以分离hmsc，联合应用免疫细胞化学、细胞化学和电镜等技术手段鉴定HMSC的方法有效。
Conclusion The HMSC can be isolated with density-grad centrifugal and anchoring screening. The method to identify HMSC with immunocytochemistry, cytochemistry and electron microscopy is effective.
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