学和免疫组织化学染色肝脏样本，从所有的动物被处理的光镜。
Histological and immunohistochemical stains Liver samples from all animals were processed for light microscopy.
方法采用常规组织学染色和免疫组织化学染色方法对家兔脊神经节内肥大细胞和P物质免疫阳性反应进行观察。
Methods Normal tissue stain and immunohistochemical stain were used to observe the mast cell and substance P immunoreactivity in dorsal root ganglia.
用多巴(DOPA)染色和免疫组织化学对细胞鉴定。
The RPE cells were identified by DOPA staining and immunohistochemistry.
方法苏木精伊红（HE）染色和免疫组织化学ABC法。
Methods Hematoxylin Eosin(HE) staining and immunohistochemical ABC method were used.
方法采用甲苯胺蓝染色和免疫组织化学方法。
Methods Using toluidine blue stains and immunohistochemical ABC methods.
方法免疫组织化学染色和图像分析技术。
Methods Immunohistochemistry technique and image analysis were used.
通过免疫组织化学方法和原位末端标记法（TUNEL）检测热休克蛋白70表达及凋亡细胞数，TTC染色观察梗死体积。
The expression of heat shock protein 70 was examined by immunohistochemistry, the apoptosis of neurons by TUNEL and the volume of cerebral ischemia by TTC staining.
方法应用免疫组织化学染色技术，以正常皮肤和正常瘢痕作对照，观察了瘢痕疙瘩和增殖性瘢痕中vegf和PCNA的表达。
Method With immunohistochemistry technique, the expression of VEGF and PCNA in keloids and hypertrophic scars was investigated, and the normal skins and scars were taken as controls.
方法免疫组织化学染色和图像分析技术。
Methods Immunohistochemical technique and image analysis were used.
方法应用免疫组织化学染色法对30例胰腺癌和10例正常胰腺组织进行VEGF表达检测。
Methods Immunohistochemical staining was used to detect the VEGF expression in 30 cases of pancreatic cancer and 10 normal pancreas tissue specimen.
方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
方法采用HE染色法和免疫组织化学ABC法。
Methods HE staining and immunohistochemical ABC method were used.
方法福尔·马林固定、石蜡包埋40例肉瘤标本进行HE和TSG101免疫组织化学染色，观察tsg101的表达情况。
Methods 40 formalin fixed and paraffin embeded sarcoma tissues were stained by he and immunohistochemistry to observe the expression of TSG101.
方法用酶联免疫吸附法（ELISA）测定血浆GMP-140水平，并用免疫组织化学染色法，观察正常肾和狼疮肾组织GMP-140表达。
Methods Plasma GMP-140 was measured with ELISA in LN patients and normal control. GMP-140 expression in the kidney was measured by a microwave-based immunohistochemistry.
方法：采用原位细胞凋亡、免疫组织化学及其双染色等技术，对70例肝细胞癌（HCC）和10例慢性肝炎的组织标本进行研究。
Methods: Expression of HCV antigens and apoptosis were demonstrated using IHC and in situ cell death detecting in samples from 70 HCC and 10 chronic hepatitis.
方法：应用he和免疫组织化学SABC法染色。
Methods: he staining and immunocytochemistry SABC methods were used.
免疫组织化学染色和免疫荧光染色提示角蛋白表达强阳性。
DOPA staining showed positive and immunohistochemical staining demonstrated that cells were stained by anti-human keratin.
免疫组织化学染色片显微镜下放大200倍，每个标本取6张切片，随机取5个视野，计算免疫组织化学染色的平均吸光度值和积分吸光度值进行半定量分析。
Under the microscope magnifying 200,6 sections of each sample in 5 random sights were selected to conduct the semi-quantitative analysis of the mean absorbance (Am) and integral absorbance (Ai).
方法：采用常规he染色光镜观察、胶体铁染色、免疫组织化学染色和透射电镜观察。
METHODS: The routine he staining, colloidal iron staining, immunohistochemistry and transmission electron microscopy were used.
应用免疫组织化学SABC染色法，对血管活性肠肽(VIP)免疫反应阳性细胞在皖西白鹅中脑和脑桥内的分布进行了观察。
Vasoactive intestinal peptide (VIP) antigen in midbrain and pons of Wanxi white goose was detected by using a immunohistochemical SABC method.
方法采用微波免疫组织化学染色（SP法）和原位分子杂交技术检测LN肾脏RANTES的表达，并进一步分析其与LN活动指数、肾脏病理和功能损害的关系。
Methods A microwave based immunohistochemistry (SP) and in situ hybridization were used to detect the expression of RANTES and renal injures in LN and normal control.
方法：利用免疫组织化学方法对大鼠牙髓组织切片进行染色，在显微镜下观察SP和CGRP免疫阳性神经纤维的分布。
Methods Colorate the slice of the rat dental pulp by the immunohistochemical methods. Observe the distribution of SP-IR and CGRP-IR nerve fibers by microscope.
方法HE染色法和免疫组织化学SABC法。
Methods he staining and immunohistochemical SABC method were used.
应用免疫组织化学染色方法，观察各组心肌梗死大鼠缺血心肌中内皮抑素、VEGF的表达和新生血管密度。
The sham group was normal control group (eight rats in each group). The expression of endostatin, VEGF and MVD in ischemic myocardium were observed by immunohistochemistry.
应用免疫组织化学染色方法，观察各组心肌梗死大鼠缺血心肌中内皮抑素、VEGF的表达和新生血管密度。
The sham group was normal control group (eight rats in each group). The expression of endostatin, VEGF and MVD in ischemic myocardium were observed by immunohistochemistry.
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