试验研究了培养液中血清的添加时间和添加浓度及不同培养体系对黄牛孤雌胚胎体外发育潜力的影响。
The effects of fetal bovine serum (FBS) addition time, concentration and different culture systems on the developmental potentiality of bovine parthenogenetic embryos were observed.
本文对禽类胚胎生物工程技术，包括禽类胚胎体外培养技术、禽类嵌合体技术和禽类基因转移技术的研究进行了综述。
The studies on biological engineering technology of embryo in birds, including the in vitro culture, chimera and transgenic birds are summarized in the essay.
免疫活性肽(ICP)和表皮生长因子(EGF)是两种生物活性因子，ICP对牛卵母细胞体外成熟及胚胎体外培养的影响还未见报道。
Immunocompentent peptide (ICP) and epidermal growth factor (EGF) are two bio - active factors, the effect of ICP on in vitro maturation of bovine oocytes and development of embryos not being reported.
目的研究低浓度氟环境对体外培养的胚胎鼠颅骨成骨细胞中的各类非胶原蛋白和胶原蛋白的表达。
Objective the expression of various non-collagenous proteins and collagen protein on the rat fetal calvarial osteoblast cultured in low doses fluoride condition in vitro had been studied.
目的研究人胚胎海马神经干细胞体外长期培养的条件和其在自主分化条件下的分化能力和分化特点。
Objective To study the conditions of in vitro culture and the features of differentiation of neural stem cells from human embryonic hippocampus.
猪卵母细胞的体外成熟培养、成熟卵母细胞的孤雌激活和胚胎培养是胚胎生物工程技术的主要环节。
The culture of porcine oocytes maturation in vitro, parthenogenetic activation of MII porcine oocytes and embryo culture are main technology of embryo biology engineering.
方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
目的从胚胎大鼠海马和上丘脑区中分离、培养神经干细胞，并进行体外增殖能力的比较。
Objective To compare the regional specificity in proliferation capacity of neural stem cells derived from hippocampus and epithalamus: study with rats.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
以CZB为基础培养液，培养小鼠4、8 -细胞胚胎单卵裂球，研究葡萄糖、牛磺酸和猪输卵管上皮细胞共培养在其体外发育中的作用。
CZB medium was used as a base medium to study the function of taurine, glucose and porcine oviductal epithelial cells system in the culture of blastomeres isolated from mouse 4? 8-cell embryos.
目的：观察脑源性神经生长因子（BDNF）和神经营养因子-3（NT-3）单独及联合应用对体外培养胚胎大鼠脊髓神经元存活和生长发育的影响。
Objective:To study the individual and joint effects of BDNF and NT-3on the sur-vival and growth of neurons from the spinal cord of embryonic rat.
旨在为研究体外孤雌胚发育和孤雌胚胎的培养提供参考。
In this paper, those factors were summed up to supply a reference to parthenogenesis embryo incubation in vitro.
方法：体外培养胚胎小白鼠小肠器官型植块，并将所有植块分为两组：实验组和对照组。
Method: Cultured the organ-type explants of mouse intestinal tissue in vitro, all the explants were divided into two groups: test group and control group.
培养滴体积和培养密度显著影响卵母细胞的成熟质量和胚胎体外发育能力。
The medium volume and density affect maturation quality of oocyte and embryonic development significantly.
目的：系统的研究小鼠胚胎干细胞的提取、体外培养扩增和向成骨细胞定向诱导分化。
Objective: research systematically into the isolation, culture and direct differentiation to osteoblast of embryonic stem cells.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
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