通过双荧光素酶报告基因测定法测定了IGFBP-3对由三碘甲状腺素刺激的生长激素启动子活性的影响。
The effect of IGFBP-3 on the growth hormone promoter activity stimulated by triiodothyronine was determined by dual-luciferase reporter assay.
结论乙酰化参与调节神经细胞中NOS1基因启动子活性。
Conclusion Acetylation may participate in the regulation of NOS1 promoter activity in neuronal cells.
方法简单、省时、可靠,可以作为一种有效的检测启动子活性的方法。
The method is simple, timesaving and dependable, thus it can be used as a kind of effective measuring in detection of promoter activity.
目的探讨血清对白念珠菌菌相转换特异基因HYR1上游启动子活性的影响。
Objective To study the effect of serum on promoter activity of Candida albicans yeast-hyphal regulational gene(HYR1).
该表达载体的构建为进一步研究人id4基因的启动子活性及表达调控奠定了基础。
The construction of expression vector of human ID4 promoter provides a platform for further researches on promoter activity and expression regulation of human ID4 gene.
目的:克隆nkx3.1基因启动子并检测其启动子活性,为研究NKX3.1基因转录调控的基本机制奠定基础。
AIM: To study the basic mechanism of transcriptional regulation, NKX3.1 gene promoter was cloned and its promoter activities in prostate cancer cell lines and other cancer cell lines were tested.
本论文工作着重进行人acat - 1基因P 7启动子活性调控和脂肪酸对人acat 1基因表达影响的两部分探索。
This work was mainly focused on the transcriptional regulation of human ACAT-1 gene P7 promoter activity, and the effect of fatty acids on human ACAT1 gene expression.
本文中我们把根系形成必需的四种PLT同源族用进化枝表示出来,启动子活性及PLT蛋白融合显示出在干细胞上最大的坡度分布。
Promoter activity and protein fusions of PLT homologues display gradient distributions with maxima in the stem cell area. PLT activities are largely additive and dosage dependent.
目的:利用已有质粒构建带GF P报告基因的启动子鉴定质粒,并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5'-UTR)启动基因表达的活性。
Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.
部分缺失的棉花曲叶病毒互补链基因启动子具有超强活性。
Partial Deleted Complementary Sense Gene Promoter from Cotton Leaf Curl Virus Shows Super Strong Promoter Activity.
启动子是基因表达调控的重要元件,启动子的活性间接反映了它所控制的基因的表达。
Promoters are important elements that regulated gene expression, activity of promoters indirectly reflect gene expression that they control.
随后人GRP94启动子克隆了荧光素酶基因上游区且缺氧环境中活性增强。
The human GRP94 promoter was then cloned upstream of the luciferase gene and showed enhanced activity in hypoxic conditions.
结果表明,获得的强启动子比原来的提高了3 ~8倍,而弱启动子则活性下降明显,其中3个几乎无活性。
Results showed that the activity of strong promoters had been improved 3 ~ 8 fold while one weak promoter had been decreased 3 fold and three weak promoters showed almost no activity.
通过对P25基因启动子的活性分析,尤其是对PS GF和BMFA调控元件的功能分析,有利于进一步了解P 25基因表达调控的精细机制。
Analysis of P25 promoter activity, especially functional analysis of PSGF and BMFA regulatory elements, could be helpful in further understanding the mechanism of P25 expression and fine regulation.
突变实验结果显示,GA盒子是小鼠RAG2启动子完整活性所必须的。
Mutant assay result showed that GA box was necessary for the full activity of murine RAG2 promoter.
PNZIP基因启动子是一个活性很高的启动子,全长启动子在叶片的启动活性是35S启动子在叶片启动活性的9倍。
The full-length promoter of PNZIP gene is a strong promoter whose GUS activity in leaves is 9 times as that of 35S.
结论IL - 6启动子可作为一种可诱导型启动子在树突状细胞内发挥活性,因而有望用于基因治疗或基因功能研究。
Conclusion IL-6 promoter can be used as an inducible promoter in researches of gene therapy and gene function.
目的探讨中国儿童正常T淋巴细胞表达和分泌的活性调节蛋白(RANTES)基因启动子- 2 8位基因多态性对儿童过敏性哮喘的影响。
Objective To study the effect of regulated upon activation, normal t cell expressed and secreted (RANTES) -28 polymorphism on allergic asthma in Chinese children.
在NOS1基因启动子区鉴定了两个正调控区域和两个负调控区域,同时TS A可上调不同长度启动子的转录活性。
Two positive regulation regions and two negative regulation regions were found within NOS1 promoter. TSA may up-regulate the transcription activities of NOS1 promoters of different length.
目的构建磷脂酰肌醇蛋白聚糖3 (GPC3)启动子荧光素酶报告基因载体,并验证其转录活性。
ObjectiveTo construct glypican-3 (GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity.
其表达方式与互补链基因启动子相似,即在叶肉及叶脉维管组织均有较高的活性。
Expression pattern of trans-activated virion sense promoter was similar to that of complementary sense promoter with the high activity in both mesophyll and vascular of leaf vein.
其表达方式与互补链基因启动子相似,即在叶肉及叶脉维管组织均有较高的活性。
Expression pattern of trans-activated virion sense promoter was similar to that of complementary sense promoter with the high activity in both mesophyll and vascular of leaf vein.
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