目的：探讨RASSF1A基因启动子区甲基化与喉癌的关系。
Objective To investigate the relationship between promoter methylation of RASSF1A gene and laryngeal squamous cell carcinoma.
方法：运用甲基化特异性P CR，检测RASSF1A基因在48例喉癌组织、相对应癌旁组织及48例正常人外周血中的启动子区甲基化情况。
Methods a methylation-specific PCR was performed to detect the promoter methylation of RASSF1A gene in 48 tumor tissues, 48 corresponding normal tissues and 48 normal blood plasma.
在启动子区域的DNA甲基化是抑制基因活性的一种重要机制。
DNA methylation in the promoter regions is a powerful mechanism for the suppression of gene activity.
这种现象主要在印迹基因和X染色体失活区域看到，这些区域中的启动子区的DNA甲基化导致了基因表达的抑制。
This phenomenon is mostly seen in imprinted genes and X-chromosome inactivation where DNA methylation of promoter regions leads to repression of gene expression.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
通常情况下，基因表达水平与DNA甲基化呈负相关，基因启动子区域甲基化从而导致该基因沉默。
Under usual conditions, there is an inverse correlation between gene expression level and DNA methylation. The gene can be silenced by DNA promoter methylation.
通常情况下，基因表达水平与DNA甲基化呈负相关，基因启动子区域甲基化从而导致该基因沉默。
Under usual conditions, there is an inverse correlation between gene expression level and DNA methylation. The gene can be silenced by DNA promoter methylation.
应用推荐