将体外翻译产物的免疫沉淀物经SDS-聚丙烯酰胺电泳,得到相当于人血清白蛋白的同位素标记的电泳区带。
When the immunoprecipitate was subjected to SDS agarose gel electrophoresis, 3 radioactive bands were obtained, one of which corresponded to pure human albumin in its position.
方法:采用同位素标记法。
同位素标记的氨基酸与普通氨基酸相比具有结构一致、性质基本相同的特点。
Compared with common amino acids, the isotope-labeled amino acids have accordant structure and analogical property.
应用同位素标记前体掺入和流式细胞分析的方法,研究CP对体外培养K 562细胞的直接作用。
The direct effect of CP on cultured K562 cells is examined in vitro by means of incorporation of isotope-labelled precursors and flow cytometry.
并可用于流式细胞术、同位素标记、单层细胞超微结构、免疫细胞化学和图像分析、原位杂交等的研究。
These methods were applicable to flow cytometry, isotope label, monolayer cell ultrastructure, immunocytochemistry, image analysis and in situ hybridization as well.
方法:以同位素标记的单克隆抗体和微球的结合率为指标。用均匀设计法研究影响免疫磁性微球构建的因素。
Methods: What determines the binding percent of monoclonal antibodies to make magnetic beads is studied by uniform design methods, isotope marked antibody's binding percent as the criteria.
结论在生长迅速的小猪模型上,尿硫测定这一无同位素标记、无创的方法可以精确反映含硫氨基酸的分解代谢情况。
Conclusions Detection of urinary sulfur, as a non-tracer and noninvasive method, may be employed to accurately measure the catabolism of sulfur amino acid in the growing piglet model.
同位素示踪研究结果显示,实验中制备的放射性同位素标记的氨基葡萄糖硫酸钠可穿过扩散池内的离体实验动物皮肤,进入皮下组织。
Isotope tracer technique showed that glucosamine sulfate above can pass through the skin in vitro of test animal in a diffusion cell and osmose into subcutaneous tissue.
以放射性同位素标记的氨基葡萄糖盐酸钠为原料制备放射性同位素标记的氨基葡萄糖硫酸钠,同时观察其穿过离体实验动物皮肤的能力。
This study prepared glucosamine sulfate with glucosamine hydrochloride marked by radio isotope and observed the osmosis ability of glucosamine sulfate passing through skin in vitro of test animal.
以放射性同位素标记的氨基葡萄糖盐酸钠为原料制备放射性同位素标记的氨基葡萄糖硫酸钠,同时观察其穿过离体实验动物皮肤的能力。
This study prepared glucosamine sulfate with glucosamine hydrochloride marked by radio isotope and observed the osmosis ability of glucosamine sulfate passing through skin in vitro of test animal.
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