结果表明,HH07 A对DNA聚合酶i催化下的DNA合成有明显抑制作用,且在一定浓度范围内存在浓度依赖性。
The results showed that HH07A strongly inhibited the DNA synthesis catalyzed by DNA polymerase I in a dose dependent manner.
应用聚合酶链反应得到2株蓝绿藻的毒素聚酮合成酶(PKS)基因,并进行基因序列分析。
Gene of polyketide synthase (PKS) from 2 toxin-producing cyanobacteria was prepared by polymerase chain reaction and the gene sequence was analyzed.
一种原核生物的dna聚合酶,具有很强的持续合成能力,执行大多数DNA的复制任务。
A prokaryotic DNA polymerase with high processivity that performs most DNA replication.
再次检查合成出的产物并更正其中错误的过程。dna聚合酶和rna聚合酶都具有一定的校正能力,以防止所合成的链中存在错误。
The process of rechecking work and correcting errors. DNA polymerase and RNA polymerase both have some ability to proofread the strands they are synthesizing.
本发明进一步提供用作分子探针或聚合酶链式反应(PCR)扩增引物的核酸分子,即合成的或天然的寡聚核苷酸。
The present invention further provides nucleic acid molecules for use as molecular probes, or as primers for polymerase chain reaction (PCR) amplification, i. e. synthetic or natural oligonucleotides.
方法:以引物互为模板的聚合酶链反应合成目的基因片段。
Methods: The PCR technique in which the primers were used as template each other was adopted to obtain the foreign DNAs for Cloning.
一般认为大肠杆菌rna聚合酶合成大肠杆菌编码乳糖代谢酶(5300 bp的乳糖操纵子)的基因的第一次转录要多长时间?
How long would it take for the E. coli RNA polymerase to synthesize the primary transcript for the E. coli genes encoding the enzymes for lactose metabolism (the 5,300 base pair lac operon)?
一般认为大肠杆菌rna聚合酶合成大肠杆菌编码乳糖代谢酶(5300 bp的乳糖操纵子)的基因的第一次转录要多长时间?
How long would it take for the E. coli RNA polymerase to synthesize the primary transcript for the E. coli genes encoding the enzymes for lactose metabolism (the 5,300 base pair lac operon)?
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