应用反复冻融法以衣藻原生质体为材料制备衣藻染色质，应用显微操作技术准确将衣藻染色质转移到烟草叶片外植体中，进行连续培养并镜检观察。
Then we exactly transformed the chromatin to the explants of the tobacco leaves by the micromanipulation, and then we cultured the explants, observed by the fluorescent microscope every day.
结果表明：枣叶片分离出的原生质体活性显著高于悬浮培养物，但是产量低于悬浮培养物分离出的原生质体。
The results showed that the viability of protoplasts isolated from leaves was significantly higher than that of the suspension culture, but the yield of protoplasts was just the opposite.
指出以叶片、愈伤组织、悬浮细胞作为分离原生质体的材料较适宜。
The isolation of protoplast, being the first step in protoplast technique, was restricted by several factors such as the resource materials, enzyme combination and osmotic condition.
主要研究结果如下：1。研究了小青杨单芽茎段培养和愈伤组织再生技术，为进一步开展离体叶片细胞染色体加倍以及原生质体培养奠定了基础。
Results are as follows: 1. Axillary buds culture and callus differentiation were studied in this paper, which provide experience for in vitro chromosome doubling and protoplast culture techniques.
主要研究结果如下：1。研究了小青杨单芽茎段培养和愈伤组织再生技术，为进一步开展离体叶片细胞染色体加倍以及原生质体培养奠定了基础。
Results are as follows: 1. Axillary buds culture and callus differentiation were studied in this paper, which provide experience for in vitro chromosome doubling and protoplast culture techniques.
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