对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.
结论重复性压迫可引起严重的骨骼肌组织的变性，特别是红肌。
Conclusion Our findings indicate that repeated compression may cause serious muscle degeneration, particularly in red muscles.
目的观测人胎盘碱性磷酸酶（HPAP）在盐酸胍变性和复性过程中构象与活力的变化，探讨其折叠机制。
Objective To examine the changes of conformation and activity of human placental alkaline phosphatase(HPAP) in denaturation and renaturation of guanidine chloride, and to study its folding mechanism.
荧光相图法研究牛血清白蛋白变性和复性过程，捕捉部分折叠中间态。
The unfolding and refolding of bovine serum albumins and the existence of intermediates were studied by fluorescence phase diagram.
蛋白变性过程中间体的存在是蛋白变性及复性动力学研究中不可缺少的证据。
The existing of intermediate in the process of protein unfolding is necessary for studying the kinetic folding and unfolding pathway of protein.
目的探讨感光受体外周蛋白结合蛋白（PBP）在体外是否有促进变性蛋白质复性的作用。
Objective The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.
本文利用荧光、紫外差光谱研究了根霉葡萄糖淀粉酶在盐酸胍变性后的复性、复活动力学。
The kinetics of the renaturation and reactivation of glucoamylase denatured by guanidine HCl was determined by means of the UV differential absorbance measurement and fluorescence emission spectra.
探讨感光受体外周蛋白结合蛋白（PBP）在体外是否有促进变性蛋白质复性的作用。
The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.
通过裂解、洗涤、变性、复性等方法对包涵体蛋白进行处理，以电泳切胶回收所获得的纯化产物作为抗原，免疫小鼠制备抗重组蛋白血清。
The recombination fusion protein was purified by lysis, washing, denaturation and renaturation. The specific band of expression was excised from the gel and used to immunize mice .
经尿素变性、梯度透析复性及去除GST“标签”后，获得重折叠的人EGFR胞内酪氨酸激酶结构域蛋白。
After denaturing in urea, gradient dialysis and cleavage of GST-tag, refolded human EGFR-TKD was obtained.
纯化后的的目的蛋白进一步复性，BMP-2经变性液溶解，装入透析袋内。
After purification, the target protein further renaturation 2 - BMP via degeneration dissolving, load dialysis bag.
当用复性缓冲液稀释还原脲变性溶菌酶时，会迅速产生可观量的沉淀。
When the denatured-reduced egg white lysozymes were diluted by the dilution refolding buffer solution, an observable amount of aggregate precipitates would immediately be formed.
当用复性缓冲液稀释还原脲变性溶菌酶时，会迅速产生可观量的沉淀。
When the denatured-reduced egg white lysozymes were diluted by the dilution refolding buffer solution, an observable amount of aggregate precipitates would immediately be formed.
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