检测橘蚜携带CTV的分子生物学反转录-聚合酶链式反应技术已建立，并已应用于检测橘蚜等蚜虫的单虫带毒情况。
Reverse transcription polymerase chain reaction technique for the detection of CTV in a single aphid or aphids has been developed.
反转录-聚合酶链反应法检测CXCR4的表达，亦得到相似结果。
Similar results were obtained through detecting the expression of CXCR4 with reverse transcription-polymerase chain reaction.
半定量反转录-聚合酶链反应验证了芯片分析结果的可靠性。
The results of semi-quantitative RT-PCR experiments supported the reliability of our microarray analysis.
采用反转录-聚合酶链反应检测两组大鼠大网膜和皮下脂肪组织中抵抗素基因的表达。
The RT-PCR was adopted to detect the expressions of resistin gene in epiploon and subcutaneous fatty tissues.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
方法提取肿瘤组织总rna，用反转录聚合酶链式反应(RT - pcr)法进行检测。
Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR.
单细胞反转录酶聚合链式反应检测胰岛素促进因子基因实验中，选择12个单个胰腺干细胞进行检测，另取12个单个未分化的干细胞作为对照。
In the detection of IPF1 gene with single cell RT-PCR, 12 single pancreatic stem cells were detected, and another 12 undifferentiated single pancreatic stem cells were taken as controls.
对40份盲样采用琼脂扩散试验(agp)、血凝抑制试验(HI)和反转录聚合酶链反应(RT - PCR)进行了检测。
40 blind samples were examined by AGAR gel precipitation (AGP), hemagglutination inhibitory (hi) test and reverse transcription-polymerase chain reaction (RT-PCR).
马传染性贫血病毒是反转录病毒科慢病毒属的成员之一，不仅与人免疫缺陷病毒具有序列同源性，而且与其血清具有交叉反应。
Equine infectious anemia virus (EIAV) is a lentivirus. It related in sequence to the human immunodeficiency virus (HIV) and reacted with acquired immunodeficiency syndrome (AIDS) patient sera.
本实验采用定量反转录聚合酶链式反应技术，观察大鼠在进行11周中等强度耐力训练过程中心脏心钠素（ANP）和脑钠素（BNP）基因表达的变化。
This study adopted RT-PCR technique to observe the change of express of gene of rats' ANP and BNP during 11 week's moderate intensity endurance training;
本实验采用定量反转录聚合酶链式反应技术，观察大鼠在进行11周中等强度耐力训练过程中心脏心钠素（ANP）和脑钠素（BNP）基因表达的变化。
This study adopted RT-PCR technique to observe the change of express of gene of rats' ANP and BNP during 11 week's moderate intensity endurance training;
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