酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
方法应用多聚酶链反应技术及限制性片段长度多态现象对46例食管癌APC和MCC基因的LOH进行了分析。
Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
目的利用直接扩增片段长度多态(DALP)这一新分子标记技术建立一套稳定的红景天属植物的DALP反应体系。
Objective Direct amplification of length polymorphism (DALP) as a new molecular marker was used to establish a set of stable DALP reaction system for the plants of Rhodiola l.
实验室鉴定方法包括聚合酶链式反应、限制酶片段长度多态性分析以及生物芯片技术等。
In laboratories, the methods mainly include polymerase chain reaction, restriction fragment length polymorphism, and biochip technology. The methods mentioned above all have their merits and defects.
方法应用聚合酶链反应限制性片段长度多态性分析方法(PCR -RFLP)对40例口腔鳞癌组织中apc基因的杂合缺失(LOH)进行检测。
Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
实验室鉴定方法包括聚合酶链式反应、限制酶片段长度多态性分析以及生物芯片技术等。
In laboratories, the methods mainly include polymerase chain reaction, restriction fragment length polymorphism, and biochip technology.
采用聚合酶链式反应（PCR）技术对来自北京等地区的173份PCV2阳性样本的全长ORF2基因进行了扩增，并对扩增产物进行了限制性片段长度多态性（RFLP）分析。
Whole ORF2 genes of 173 positive clinical PCV2 samples from Beijing and other areas were amplified by PCR and followed by restriction fragment length polymorphism (RFLP) analysis.
方法在140名汉族健康人的外周血中，应用聚合酶链反应（PCR）限制性片段长度多态性分析（RFLP）及多重PCR技术，进行NAT1等位基因分型研究。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
用S基因聚合酶链反应-限制性片段长度多态性确定HBV基因型。
HBV genotypes were determined by RFLP based on S-gene PCR products.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
方法应用聚合酶链反应限制性片段长度多态性技术检测296例两个基因多态位点的等位基因、基因型。
MethodGenotypes and alleles of polymorphisms of both genes were determined with polymerase chain reactionrestriction fragment length polymorphism assay (PCRRFLP) of 296 subjects in Han Chinese.
聚合酶链反应-限制性片段长度多态性法；
Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP);
采用多聚酶链反应-限制性片段长度多态性法(PCR - RFLP)分析MGP和ALAD基因的多态性。
The polymorphisms of MGP gene and ALAD gene were analyzed by the methods of PCR-RFLP.
采用病例对照研究的方法，用聚合酶链反应(PCR)和限制性片段长度多态性分析(RFLP)研究TS患者与健康对照间DRD2P、COMT基因的基因型频率有无统计学差异。
DRD2P and COMT genotyping were carried out using PCR followed by RFLP and statistical analysis of genotype frequencies between ts patients and healthy controls was performed using SPSS programme.
方法：运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR -RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
方法：选择符合入选标准的高血压患者336例，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的方法，进行ACE2基因分型。
Methods:336 hypertension patients were recruited in this trail. The distribution of ACE2 gene A9570G polymorphism was analyzed by PCR-RFLP in all participants.
方法：选择符合入选标准的高血压患者336例，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的方法，进行ACE2基因分型。
Methods:336 hypertension patients were recruited in this trail. The distribution of ACE2 gene A9570G polymorphism was analyzed by PCR-RFLP in all participants.
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