主要是将第二、三轮的嵌套扩增中的线性延伸单独列出，从而增加了反应的特异性和高效性。
The linear extension of the second and three rounds of nested amplification had been listed separately in improved system, it would increase the specificity and efficiency of PCR reaction.
主要是将第二、三轮的嵌套扩增中的线性延伸单独列出，从而增加了反应的特异性和高效性。
The linear extension of the second and three rounds of nested amplification had been listed separately in improved system, it would increase the specificity and efficiency of PCR reaction.
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