反向引物SA4或EM6与研究的所有正向引物组合时产生的多态性条带分子量完全相同，这些条带可能是由反向单引物扩增而来的。

The reverse primers SA4 or EM6 produced identical polymorphic bands when combined with all the forward primers tested. These bands might be amplified by the reverse primers.

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对其阳性克隆扩大培养，大量提取质粒，以T7，SP6为测序引物进行全自动正、反向测序确认。

T7 and SP6 were taken as sequencing primers for forward and backward sequencing identification automatically.

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对其阳性克隆扩大培养，大量提取质粒，以T7，SP6为测序引物进行全自动正、反向测序确认。

T7 and SP6 were taken as sequencing primers for forward and backward sequencing identification automatically.

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