测定TRP - 1反义核酸转染黑素细胞后细胞内黑素含量的变化。
Then, the content of melanin in antisense TRP-1 transfected melanocyte was detected.
反义核酸药物是基因药理学的一个新领域，对于肿瘤、遗传病以及传染病的防治具有重要意义。
As a new territory of genetic pharmacology, antisense nucleic acid drug has been promoting the development of tumor research as well as the study of genetic and infectious diseases.
结论CD147反义核酸能抑制卵巢癌的转移和成瘤，因而有可能成为卵巢癌的治疗的药物靶点。
CONCLUSION CD147 antisense RNA can inhibit invasion and tumorigenesis of ovarian cancer cells and CD147 may become a new drug target of tumor therapy.
肽核酸(PNA)作为一种反义核酸类化合物，在分子水平的疾病诊治上，有其独特的优越性，现在日益受到关注。
PNA, which is one kind of anti sense nucleic acid compounds, has its unique advantages in the molecular diagnostics and treatment of diseases. Now, people gradually attach more importance to PNA.
反义核酸作为一种新的抗病毒、抗肿瘤药物，它的应用受到越来越多的重视，但反义核酸技术的研究也面临一些挑战。
As a new anti - virus, anti - tumor drug, antisense nucleic acid's application has been given more and more attention, but the research of antisense nucleic acid technology also faces some challenges.
方法在小鼠角膜肿瘤模型上，测定两种VEGF反义核酸序列的5种不同修饰之反义核酸，对肿瘤血管形成和肿瘤生长的抑制作用。
Methods with a tumor model on the cornea of rat the inhibition of angiogenesis and tumor growth by 5 ODNs with different modifications and sequences was determined.
方法：采用TRAP法检测K 5 6 2细胞在反义核酸处理前后端粒酶活性的变化，以及采用TUNEL法观察反义核酸处理前后细胞凋亡的变化。
Methods: Telomerase activity of K562 cells with antisense treatment was measured using the TRAP assay, and apoptosis was studied by means of TUNEL method.
此外，鉴于肽核酸独特的理化和生物学性能，人们正尝试将其用于生物分子工具、反义和反基因制剂、分子探针和生物传感器。
In addition, the unique chemical, physical and biological properties of PNAs have been exploited to produce powerful biomolecular tools, antisense and antigene agents, molecular probes and biosensors.
成功的反义显像要求反义寡核苷酸易被细胞摄取、耐核酸酶、杂交稳定、标记简单。
The requirements for ideal antisense imaging include easy intakeing by cells, convenient labelling, stable hybridization and the tolerance against nuclease.
由于他们能与信使核糖核酸(mRNA)靶特异性结合，进而抑制靶基因的翻译，因而在反义技术中已被成功地应用。
They have been successfully used in antisense technology, for their ability to specifically bind to messenger RNA (mRNA) targets and to inhibit translation of the target genes.
由于他们能与信使核糖核酸(mRNA)靶特异性结合，进而抑制靶基因的翻译，因而在反义技术中已被成功地应用。
They have been successfully used in antisense technology, for their ability to specifically bind to messenger RNA (mRNA) targets and to inhibit translation of the target genes.
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