目的构建大肠杆菌双杂交系统的诱饵重组质粒。
Objective To construct a bait plasmid in bacterial two-hybrid system.
目的构建DLX4酵母双杂交系统并鉴定其自激活作用。
Objective To construct a yeast-two hybrid system of DLX4 and identify its self-activation.
细菌双杂交系统是新近建立的一种研究蛋白质间相互作用的方法。
Bacterial two-hybrid system is a newly developed method for studying protein-protein interaction.
目的：检测抗增殖蛋白在酵母双杂交系统中是否具有转录自激活作用。
Objective: To assay the transcriptional activation effect of prohibitin in yeast two hybrid system.
结论应用酵母双杂交系统，获得了一些候选的NECL1胞内区相互作用蛋白。
Conclusion Using yeast two-hybrid system, we got some alternative proteins which may interact with the cytoplasmic tail of NECL1.
采用酵母双杂交系统，我们用LNX蛋白中的PD Z结构域为诱饵，筛选相互作用蛋白。
Using the PDZ domain in LNX protein as bait, we screened for LNX-interacting proteins by yeast-2-hybrid system.
但是巨大的蛋白质网络比我们想象要大得多，单一的双杂交系统不能解决所有问题，需要同其它的方法有效地结合。
Nevertheless, huge protein network is larger than that we predict and single yeast two-hybrid system cannot solve all the problems, which need be complemented by other wags.
利用酵母双杂交系统对4种天然和合成的雌激素，4种植物性激素以及14种常用于自来水供水系统中的酚类物质进行了内分泌干扰作用的筛检测定。
The endocrine disrupting functions of 4 natural and synthetical estrogens, 4 phytoestrogens, 14 phenols widely used in the tap water supply system were detected using yeast two-hybrid technique.
前言： 近年来，一些不依赖于转录因子活性的新型双杂交系统相继建立，如分离的泛素系统、 蛋白质片段互补分析、阻遏物重构分析和SOS恢复系统等。
Some new type of two-hybrid systems, such as split-ubiquitin system, protein-fragment complementation assay, repressor reconstitution assay and SOS recruitment system, have been developed recently.
结论利用酵母双杂交Gal4系统3研究与HCV核心蛋白相互作用的蛋白，证实了HCV核心蛋白无自激活功能。
CONCLUSION: Yeast two-hybrid GAL4 system 3 can be utilized to fish HCV core region-interacting protein. HCV core protein does not have self-activating function.
结论利用酵母双杂交Gal4系统3研究与HCV核心蛋白相互作用的蛋白，证实了HCV核心蛋白无自激活功能。
CONCLUSION: Yeast two-hybrid GAL4 system 3 can be utilized to fish HCV core region-interacting protein. HCV core protein does not have self-activating function.
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