利用双层平板法和微孔快速筛选法，共筛得三株有活力的菌株，对其中活力较好的一株进行了深入研究。
Totally, three strains which had higher enzyme activity were obtained by double-plate and rapid micro-bacteria methods. The one showed highest enzyme activity was selected to do further study.
建立了简便的双层平板诱导法对启动子进行初筛。
We have constructed suitable screening method for low-temperature sensitive promoters.
稳定剂为琥珀酸钠，再生培养方法为平板双层法。原生质体形成率和再生率分别达到93.8%和37.5%。
The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.
稳定剂为琥珀酸钠，再生培养方法为平板双层法。原生质体形成率和再生率分别达到93.8%和37.5%。
The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.
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