目的： 建立人骨肉瘤蛋白质组双向电泳图像分析方法。
To establish analysis methods of two-dimensional (2-DE)gel electrophoresis for human osteosarcoma.
蛋白质组研究技术主要包括双向电泳、质谱和生物信息学。
Proteomic techniques mainly include two-dimensional electrophoresis, mass spectrometry and bioinformatics.
方法用双向电泳方法比较了3例正常肝和8例HCC核基质蛋白成分。
Methods Using high resolution two dimensional polyacrylamide gel electrophoresis, the nuclear matrix proteins of 3 normal livers and 8 HCC were compared.
应用双向电泳技术对果梅完全花与不完全花的蛋白质组分进行了比较分析。
Two-dimensional electrophoresis was used to analyze differential proteins between the perfect and imperfect flowers in Japanese apricot(Prunus mume Sieb. et Zucc).
目的探索可兼顾双向电泳凝胶图像质量和结果保真性的软骨蛋白提取方案。
Objective To explore an optimal cartilage protein extraction approach that can guarantee both the image quality and the result fidelity of the two-dimensional gel electrophoresis (2-DE) technique.
依赖双向电泳的杂交检测单元，配备该检测单元的传感器芯片，和杂交检测的方法。
Hybridization detecting unit relying on dielectrophoresis, sensor chip provided with the detecting unit, and method for detection of hybridization.
借助双向电泳、质谱分析等蛋白质组学分析方法，构建斑马鱼胚胎的蛋白质表达谱。
In addition, by means of two-dimensional electrophoresis, mass spectrometry and other proteomic methods to construct zebrafish embryo protein expression profile in different periods.
【目的】探索体外培养星形胶质细胞双向电泳样品制备方法，提高电泳分辨率和重复性。
To explore the methods of sample preparation of two-dimensional electrophoresis of astrocyte cultured in vitro, for improving its resolution and reproducibility.
方法用SDS-PAGE和双向电泳方法，对日本血吸虫两性成虫全虫可溶性抗原进行分析。
Methods The soluble antigens of the parasites were analysed by means of SDS PAGE and two dimensional gel electrophoresis.
双向电泳技术作为高效、高分辨率的蛋白质分析手段，在现代生命科学研究中扮演着重要的角色。
Two dimensionl electrophoresis plays a key role in modern life technology as a high efficiency and high resolution means of protein analysis.
目的：初步建立人晶状体蛋白质组研究中的双向电泳分离技术，提高晶状体蛋白分辨率和重复性。
Objective:To establish and optimize two-dimensional electrophoresis (2-DE) for proteonomic analysis of the human lens and to promote resolution and reproducibility.
目的建立正常人和矽肺人群血清双向电泳图谱，寻找矽肺血清差异蛋白，进一步探索其发病机制。
Objective To establish 2-dimensional gel electrophoresis (2-de) images and seek differentially expressed serum proteins for understanding the pathogenesis of silicosis.
方法：采用双向电泳免疫印迹检测法分析了83例CHD患者血清hdl亚类组成及相对百分含量。
Methods: The contents of serum HDL subclasses in 83 CHD subjects were determined by two dimensional gel electrophoresis associated with immunodetection method.
通过对传统的双向电泳方法进行改进与优化，得到了一种适合于分析植物叶片蛋白质的双向电泳新方法。
Based on the method of o Farrell, an improved procedure for the two dimensional gel electrophoresis of leaf proteins is presented.
目的建立和优化终末期肾病患者血清蛋白质组研究的双向电泳及相关技术，并与正常血清蛋白图谱比较。
Objective To establish and optimize the two-dimensional gel electrophoresis technical platform for the blood serum proteome research in patients with end stage renal disease(ESRD).
通过对双向电泳图谱的初步观察可以看到，蛋白质主要集中在等电点5—8和分子量28kd—66 KD之间。
From 2-d electrophoresis maps, we can find that most of proteins focused between pI5 - pI 8 and molecular weight 28kd-66kd.
结果双向电泳图谱上有6个点在两组间存在明显差异，4个蛋白质点在GBS疾病组中表达明显上调，2个降低。
ResultsThere were 6 protein spots that had significant differences in expression between the two groups. Expressions of 4 protein spots in the GBS group increased and 2 decreased.
肿瘤患者脑脊液和正常对照脑脊液双向电泳图谱有明显差别，但是本实验尚未对这些差异蛋白进行质谱鉴定，所以未找出肿瘤特异蛋白。
There are obvious difference between the two 2D gels, but the different protein spots are not identified by mass spectrum, so the tumor special protein is not found.
采用双向电泳技术分析了正常大鼠、心肌缺血模型大鼠、药物处理后的大鼠的血清蛋白组的差异，从而探讨利用双向电泳技术分析药物处理的有效性。
So in this paper we adopt 2D-PAGE to analysis the different serum protein group of healthy rat , cardiac muscle bloodless rat, treated drug-taken rat so that we may discuss the medication effect.
用pH3 -10的IPG胶条进行双向电泳，经考马斯亮蓝染色后，可在胶面上检测到大约450个蛋白点，其中约有89%的蛋白是酸性蛋白。
Stained with coomassie brilliant blue, nearly 450 protein spots in a 2-de gel were detected with the pH 3 ~ 10 gradient IPG strip, among which about 89% proteins were acidic ones.
用pH3 -10的IPG胶条进行双向电泳，经考马斯亮蓝染色后，可在胶面上检测到大约450个蛋白点，其中约有89%的蛋白是酸性蛋白。
Stained with coomassie brilliant blue, nearly 450 protein spots in a 2-de gel were detected with the pH 3 ~ 10 gradient IPG strip, among which about 89% proteins were acidic ones.
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