目的制备多种抗猪鼻支原体的单克隆抗体，建立双抗体夹心ELISA方法用于该病原体的检测。
Objective To prepare monoclonal antibodies(McAbs) of Mycoplasma hyorhinis and establish an antibody-sandwich ELISA method for the detection of the organism.
方法：采用单克隆与多克隆双抗体夹心法测定血清白介素-2 (IL - 2)及可溶性白介素- 2受体(SIL - 2r)水平。
Methods: Monoclonal and poly - clonal dual antibody sandwich method was used to assay the levels of serum interleukin - 2 (IL - 2) and soluble interleukin - 2 receptor (SIL - 2r).
采用多克隆双抗体夹心elisa法快速检测狂犬病毒抗原含量。
The rabies virus antigen contents were detected by double antibody sandwich ELISA assay using polyclonal antibody.
以单克隆抗体4c2作为捕捉抗体，兔多克隆抗体作为检测抗体，建立了ELISA双抗体夹心法，检测RV抗原。
A double antibody sandwich ELISA for RV antigen was developed using McAb 4c2 as capture antibody and rabbit PcAb as detection antibody.
结论：双功能抗体的有效率明显高于单克隆抗体。
Conclusion: The effectiveness of BsAb was significantly higher than that of monoclonal antibodies...
采用免疫双扩法鉴定单克隆抗体类型，ELISA法、免疫双扩法鉴定单克隆抗体的特异性。
Double immunodiffusion assay was used to identify the type and subclass of monoclonal antibody. ELISA and double immunodiffusion assay were used to determine the specificity.
采用免疫双扩法鉴定单克隆抗体类型，ELISA法、免疫双扩法鉴定单克隆抗体的特异性。
Double immunodiffusion assay was used to identify the type and subclass of monoclonal antibody. ELISA and double immunodiffusion assay were used to determine the specificity.
应用推荐