方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法：采用逆转录-聚合酶链反应(RT - PCR)检测48例胃癌组织及30例正常组织中vegf基因的表达。
Methods: The expression of VEGF gene in 48 samples of human gastric carcinomas and 30 samples of corresponding normal tissue specimens was detected by RT-PCR.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
方法：建立DI大鼠模型，应用逆转录-聚合酶链反应(RT - PCR)方法检测DI及逼尿肌稳定(DS)大鼠逼尿肌组织IP3R亚型变化。
Methods: To set up the model of DI rats, the IP3R isoforms expressions of detrusor tissue in DI rats and ds rats were detected by RT-PCR technique.
方法：应用逆转录-聚合酶链反应(RT - pcr)法检测73例急性白血病患者及23例正常人的WT 1及LRP基因的表达。
Methods: Expressions of WT1 and LRP genes were measured in 73 patients with acute leukemia and 23 normal controls by RT-PCR method.
方法采用逆转录聚合酶链反应（RT PCR）方法检测5 2例肺癌组织及远癌正常肺组织SEMA3B基因产物的表达水平。
Methods The expression of SEMA3B was detected in 52 human lung cancer tissues and paired 52 non cancer tissues by To explore the relationship between SEMA3B gene expression and onc RT PCR.
方法采用逆转录聚合酶链反应（RT PCR）方法检测5 2例肺癌组织及远癌正常肺组织SEMA3B基因产物的表达水平。
Methods The expression of SEMA3B was detected in 52 human lung cancer tissues and paired 52 non cancer tissues by To explore the relationship between SEMA3B gene expression and onc RT PCR.
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