方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
采用双标记流式细胞分析技术，分别检测各组小鼠脾脏及肠系膜淋巴结（MLN）CD80M和CD86 M的表达。
The number of CD80/CD86 M of spleen and mesenteric lymph node(MLN) were determined by double-label FCM method.
方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。
Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.
方法分别采用流式细胞技术和散色比浊法测定HSP急性期与恢复期及对照组儿童血清免疫球蛋白浓度和T淋巴细胞亚群水平。
Methods The immunoglobulin concentration and T-cell subgroup level of acute stage and convalescent stage HSP children were detected with FCM and nephelometer.
并通过流式细胞仪和激光共聚焦显微镜等技术探讨了聚合物在细胞水平的靶向能力及靶向机理。
As revealed by flow cytometry and confocal microscopy, the dendrimer-biotin conjugate exhibits exhibited much higher cellular uptake into cancer cells than the conjugate without biotin.
方法采用酶直接消化法从胎盘组织中提取间充质干细胞，并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。
Methods the enzyme directory digestion method was used to isolate Mesenchymal stem cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells.
方法采用酶直接消化法从胎盘组织中提取间充质干细胞，并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。
Methods the enzyme directory digestion method was used to isolate Mesenchymal stem cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells.
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