方法：应用pcrSSCP及免疫组织化学方法对比研究。
Methods Make a comparison between PCR SSCP and immunohistochemical method.
方法：采用大鼠慢性应激抑郁模型、灌胃给药、行为学及免疫组织化学方法。
METHOD: This study adapted the depression rat model of chronic stress, behavior and immunohistochemistry.
方法观察24例透明细胞肉瘤的病理形态及免疫组织化学特征。
Methods The pathomorphology and immunohistochemistry features were observed in 24 cases of clear cell sarcoma.
方法：用免疫组织化学方法对小鼠胸腺s100蛋白的表达时间、表达强弱及分布进行原位观察。
Methods: By immunohistochemical method the time, intensity and distribution of the expression of S100 protein was observed in situ.
应用免疫组织化学abc方法，检测52例卵巢浆液性良、恶性及交界性肿瘤中egf、EGF - R的表达。
By using immunohistochemical ABC method, the expression of EGF and EGFR in 52 patients with various serous tumors of the ovary was examined.
方法：采用免疫组织化学方法和图像分析技术观察adam28在小鼠牙胚发育各期的表达分布及差异。
Methods: Immunohistochemistry and image analysis technique were used to observe the expressions of ADAM28 at mouse tooth germ development stages.
免疫组织化学方法法显示一氧化氮合酶的表达，用光学显微镜在高倍镜下观察，识别黑素细胞，观察细胞胞质的染色及特点。
Expression of NOS was assayed with immunohistochemical method and melanocyte was identified under high-times optic microscope to observe stain and characteristic of cytoplasm.
利用免疫组织化学方法及图像分析系统对各组动物脊髓内星形胶质细胞的时空分布和变化进行观察和分析。
Immunohistochemical technique and imaging analysis technique were used to detect the temporal and spatial distributions and changes of the astrocyte in spinal cord in the animals of each group.
方法：用免疫组织化学方法对36例肾盂癌及癌旁组织研究，并和7例正常肾盂组织进行对照。
Methods:The samples from 36 pelvic carcinomas and their pericarcinous tissues were detected by immunohistochemical technique, and 7 normal pelvic tissues were as controls.
通过免疫组织化学方法和原位末端标记法（TUNEL）检测热休克蛋白70表达及凋亡细胞数，TTC染色观察梗死体积。
The expression of heat shock protein 70 was examined by immunohistochemistry, the apoptosis of neurons by TUNEL and the volume of cerebral ischemia by TTC staining.
方法应用免疫组织化学法(SP方法)，检测15例正常卵巢组织，15例卵巢良性肿瘤组织及60例卵巢癌组织中egfr的表达情况。
Methods Immunohistochemistrical SP method was used to detect the expression of EGFR in 15 cases of normal ovarian tissue and 15 cases of benign ovarian tumor and 60 cases ovarian cancer.
用PRV和5 -HT免疫组织化学双标记方法研究脑内5 - HT能神经元对咽肌的神经支配及调控。
Innervation and control of 5 HT neurons to the pharyngeal muscle were studied using PRV and 5 HT immunohistochemical double labeling method.
方法：应用免疫组织化学方法测定42例，HCC标本和癌旁标本中P27及PCNA的表达。
Methods:P27 and PCNA were detected in 42 HCC specimens and paracarcinoma specimens and paracarcinoma specimens by immunohistochemical technique.
方法对40例手术切除的原发性食管癌标本进行免疫组织化学染色，确定其VEGF的表达及微血管密度。
Methods Surgical specimens from 40 primary esophageal carcinoma were examined for VEGF expression and microvascular density by immunohistochemical staining.
方法应用免疫组织化学方法检测45例鼻咽癌组织及20例鼻咽黏膜不典型增生组织中COX- 2的表达。
Methods The expression of COX-2 was detected by immunohistochemical method in specimens from 45 NPC patients and 20 cases of nasopharyngeal atypical hyperplasia.
方法应用免疫组织化学方法对43例宫颈癌及癌旁组织中的MMP2、MMP9、TIMP1和TIMP2进行检测。
Methods Immunohistochemicall method was employed to detect the expression of MMP2, MMP9, TIMP1 and TIMP2 in 43 case of cervical carcinoma.
应用免疫组织化学方法对正常胰岛及52例胰岛细胞内分泌肿瘤中第x因子样抗原进行观察。
The factor X-like antigen in normal pancreatic islets and in 52 cases of pancreatic endocrine tumours was investigated by immunohistochemistry.
方法：用免疫组织化学abc法及图像分析技术对沙土鼠脑缺血海马CA 1区5 - HT含量变化进行研究。
Methods: The change of 5-ht in CA1 sector of hippocampus in gerbils following cerebral ischemia and reperfusion was examined by ABC method of immunohistochemistry and image analysis technique.
方法免疫组织化学方法(SABC法)示s 100蛋白阳性细胞及GFAP阳性细胞。
Methods Immunohistochemical method (SABC method) was applied to exhibit the S100-IR and GFAP-IR cells.
方法：采用HE染色法及免疫组织化学abc法。
Methods: he staining and immunohistochemical ABC methods were used.
方法取正常和先天性畸形人胚心脏，采用免疫组织化学及图像分析技术对心脏内hcy2表达变化进行定量测定分析。
Methods Immunohistochemistry and the technique of picture assay were used to detect HCY-2 expression in the normal and abnormal human embryonic heart.
方法采用免疫组织化学方法，检测42例喉癌组织中vegf与MMP - 9表达及肿瘤微血管计数(mvc)。
Methods VEGF, MMP-9 and microvessel count (MVC) were detected by immunohistochemical method in 42 laryngeal carcinoma specimens.
方法：用s -P免疫组织化学方法分别检测71例肝细胞性肝癌(HCC)15例肝硬化及10例正常肝脏pten蛋白表达。
Methods 71 cases of hepatocellular carcinoma (HCC), 15 cases of hepatocirrhosis, 10 cases of normal liver were selected for PTEN protein expression by S-P immunohistochemistry.
方法采用免疫组织化学方法及光镜观察成年大鼠肠道各段IEL与LPL的形态和分布特征。
Methods Used immunohistochemistry methods and electron microscope to observe the shape and the distribution of IEL and LPL which come from the mature rat.
方法：以71例不同级别的人胶质瘤组织为研究材料，用免疫组织化学染色方法检测端粒结合因子TRF1及TRF2蛋白。
Methods:The expression of TRF1 and TRF2 proteins in 71 human gliomas specimens with different malignant grades were studies using immunohistochemistry methods.
方法石蜡包埋切片，应用免疫组织化学SP法及麦克奥迪真彩色病理分析图像分析系统，对TTF-1表达强度的阳性单位（PU）进行定量分析。
Methods The TTF-1 expression intensity (in positive units, PU) in paraffin-embedded tissue blocks was determined using immunohistochemical SP method in combination with Motic Images System.
用免疫组织化学方法检测MAP-2和NF表达，比较不同缺血程度及不同再灌注时间MAP-2和NF表达的改变；
The expression of MAP-2 and NF were detected by immunohistochemistry (ABC methods) and the intensity of immunostaining was measured using MedHPIAS pathologic imaging-analysis system.
方法用HE及SP法免疫组织化学染色对5 2例形态学变异的纤维结构不良作回顾性分析。
Methods HE staining and immunohistochemical staining technology (S-P method) were used to do retrospective analysis for 52 cases of fibrous dysplasia, which had morphologic abnormalities.
方法应用免疫组织化学方法对122例晚期NSCLC组织进行ERCC1、RRM1及BRCA1蛋白表达检测。
Methods The expression of ERCC1, RRM1 and BRCA1 protein in 122 cases of advanced NSCLC was detected by immunohistochemical methods.
方法：采用免疫组织化学方法及电镜法，观察成年大鼠肠道各段IEL与LPL的形态和分布特征。
Methods:Immunohistochemistry and electron microscope were used to observe the shape and the distribution of IEL and LPL which come from mature rat.
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