的确，去核的卵细胞在这个过程中扮演着至关重要的角色——可是具体机制是什么？
It's sure that enucleated egg cell plays an important role in the process — then, what is the concrete mechanism?
供体细胞核移入去核的卵母细胞后，必定要经过表观遗传修饰的重编程过程，回到胚胎开始发育的全能状态。
The donor nucleus must experienced the epigenetic modification of the process reprogramming and went back to the initial state after the donor cell was injected into the oocytes.
蛋白质磷酸化和去磷酸化作为原核和真核细胞表达调控的关键环节，了解其对功能的影响可以深入理解生命系统在分子水平的调控状况。
As the key point of expression modulation in prokaryotic and eukaryotic cells, the protein phosphorylation and dephosphorylation may help reveal the status of the life system at the molecular level.
改进的小鼠表面张力辅助去核法是一种适用于小鼠卵母细胞操作的快速、简单、实用的去核方法。
Modified surface tension assisted method is a efficient and simple enucleation method with the advantage of saving manipulating time and non-damage to mouse oocyte.
本文结果证明，外源DNA能在去核卵细胞中诱导核重构的发生。
The results supported the earlier conclusion that exogenous DNAmight induce nuclear reconstitution in the eggs.
目的探讨新的小鼠卵母细胞透明带膨胀（ZPD）辅助去核法的可靠性。
Objective: To study the reliability of the new enucleation method for mouse oocyte: zona pellucida dilating (ZPD) assisted enucleation method.
去核的细胞质仍然能合成蛋白质。
研究小鼠红细胞分化去核分化因子（MEDDF）在红细胞终末分化中的功能。
Objective To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), newly cloned in our laboratory, in erythroid terminal differentiation.
方法：用碱性磷酸酶抗碱性磷酸酶法检测再障患者骨髓中T淋巴细胞表型的变化，并用半固体培养法检测再障患者骨髓及去T细胞后骨髓单个核细胞CFU-GM集落形成情况。
Methods: Phenotype of T lymphocytes in bone marrow and CFU GM yields of bone marrow mononuclear cells with or without T cells were assayed with APAAP and semi solid culture method, respectively.
用离子霉素或乙醇预激活去核卵母细胞，对重组胚的卵裂和发育无明显影响。
Preactivating donor cells and enucleated oocytes with ionomycin has no significant enhancive effect on the cleavage of constructed embryos, but it arrests the blastocyst development.
去核时卵母细胞质剩得过少可导致移核胚过早（6-细胞期）发生致密化或形成内细胞团细胞数目较少的小囊胚；
Too much reduction of the cytoplasmic mass at enucleationled to premature compaction of the nuclear transplant embryos at 6-cell stage or the formation of small blastocysts with few ICM cells;
为了确保细胞的去核，使用了紫外光检查位于极化体和中期板中的DNA。
To make sure the enucleation, ultraviolet light is used to check DNA located in the polar body and the metaphase plate.
为了确保细胞的去核，使用了紫外光检查位于极化体和中期板中的DNA。
To make sure the enucleation, ultraviolet light is used to check DNA located in the polar body and the metaphase plate.
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