洋葱鳞茎表皮细胞内可见皮层微管，呈薄片状，原生质体染色阴性。
Cortical microtubules were observed in intraepidermal cells of onion bulb with a thin section and negative staining of protoplasts.
合子原生质体CTC荧光呈极性分布。
The CTC fluorescence was polar distributed in the zygote protoplast.
原生质体在培养基中诱导出小愈伤组织。
为嗜杀酵母原生质体融合育种提供了依据。
It provides scientific basis for the breeding of protoplast fusion of killer yeast.
温度明显影响原生质体融合的频率和速度。
Temperature remarkably affected the frequency and the speed of protoplast fusion.
所得双亲菌株原生质体的融合率为3.31%。
The fusion rate of the inactivated parental protoplasts was 3.31%.
这是迄今第一个由籼稻原生质体再生植株的报道。
This is the first report of plant regeneration from the protoplast culture of an indica rice.
随纤维素酶浓度升高，原生质体产量及活力增大。
Yieldand viability of mesophyll protoplasts were increased as Cellulase concentration was increased.
系统建立的基础性工作：原生质体培养与生理研究。
Basic work for the system establishment about protoplast culture and physiological research.
接触的细胞的原生质体通过原生质连丝连接成一体。
The protoplasts of attached cells are connected and integrated by plasmodesmata.
对原生质体的浅层液体培养和固体培养进行了比较。
The thin liquid culture was compared with that of the AGAR plate.
利用双灭活原生质体融合技术获得了苏云金杆菌新菌株。
The inactivated protoplast fusion method was carried out to select new Bacillus thuringiensis strains.
研究了啤酒酵母原生质体融合株GR8的主要发酵特性。
The fermentative characters of the brewer, s yeast protoplast fusant GR8 were studied.
本文研究的是一种通过原生质体融合技术构建出的细胞工程菌。
In this paper, the strain is cell engineering bacteria, which constructed by the way of protoplast fusion.
芦笋的花药、花粉、茎尖及原生质体经离体培养均可再生植株。
The anther, pollen, stem point and bioplast cultured in vitro can be plant.
油菜已经建立了子叶、胚轴、原生质体及小孢子培养的再生体系。
Rape have established regeneration system of cotyledons, hypocotyls, protoplasts and microspore culture.
马铃薯叶肉细胞原生质体培养后，再生细胞形成细胞团和愈伤组织。
The mesophyll cell protoplasts of potato could divide from cell colonies and callus after cultured.
蜗牛酶和1.5％溶壁酶酶解金针菇菌丝获得了高产量的原生质体。
High yield of protoplasts has been obtained by using enzyme containing 1% snailase or 1.5% lywallzyme.
对原生质体的分离、培养、融合及植株再生方面进行了简要的论述。
The isolation, culture and fusion technology of plant protoplast have been developing rapidly over the past twenty years.
预分化实验表明：ABA能有效地提高原生质体愈伤组织的再生能力。
Predifferentiation showed that ABA was effective to increase the frequency of regeneration of protoplast-derived calli.
研究结果对建立高效的龙眼原生质体再生系统及其遗传操作有参考价值。
This study would be of value for establishing high-effective regeneration system of protoplasts and the related genetic manipulations in longan.
同时，对原生质体再生菌株的形态与多杀菌素产量变化进行了比较研究。
In addition, we compared the change of morphology and spinosyns yield of the regenerated strains.
大豆因其愈伤组织难以分化、原生质体再生困难等因素，其再生体系一直不够完善。
The regeneration system of soybean is not perfect because the differentiation of callus tissue and the protoplast regeneration are difficult.
研究结果表明，香菇原生质体分离诱变是一种很有应用价值的食用菌菌种选育方法。
The results indicated that protoplast formation and mutagenesis are the potential methods for strain selection and improvement in edible fungi.
原生质体是植物遗传改良的重要实验体系之一，而原生质体的分离是该体系的基础。
Protoplast is an important system for plant genetic improvement, and the isolation of protoplast is an essential step for the protoplast manipulation.
研究了球形红假单胞菌与酿酒酵母菌的原生质体融合条件，并对融合子性能进行了测定。
The results of the protoplast fusion between Rhodopseudomonas spheroides and Sacchaaromyces cerevisiae were studied in this paper.
结果表明，外源基因转化球孢白僵菌原生质体时，可以以抗除草剂基因为转化筛选标记。
The results indicated that herbicide resistant gene should be used as a selection marker gene in protoplast genetic transformation of B. bassiana.
结果表明，外源基因转化球孢白僵菌原生质体时，可以以抗除草剂基因为转化筛选标记。
The results indicated that herbicide resistant gene should be used as a selection marker gene in protoplast genetic transformation of B. bassiana.
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