禾长蠕孢菌原生质体转化及突变体筛选与验证。
Transformation of HGE protoplasts and screening of the mutants.
糖多孢红霉菌的原生质体转化和染色体同源整合，是红霉素生物合成基因改造的重要途径。
Protoplast transformation and homologous chromosomal integration of s erythraea was an important path in changing gene involved in erythromycin biosynthesis.
对7号淀粉酶菌M1033的遗传背景进行分析，建立了其原生质体制备、转化的优化条件。
After Genetic background analysis of Streptomyces diastaticus No. 7 strain M1033, the modified conditions of M1033 protoplasts and transformation were established.
现代育种手段进行果酒酵母的选育方法有诱变育种、杂交育种、原生质体融合、基因克隆和转化等技术。
The breeding methods of fruit wine yeast nowadays cover mutation breeding, cross breeding, protoplast fusion, and genetic cloning and transformation etc.
结果表明，外源基因转化球孢白僵菌原生质体时，可以以抗除草剂基因为转化筛选标记。
The results indicated that herbicide resistant gene should be used as a selection marker gene in protoplast genetic transformation of B. bassiana.
红麻的器官培养和原生质体培养是品种快繁、变异体筛选以及体细胞杂交和遗传转化等生物技术育种的基础。
It's organ culture and protoplast culture is important basic technique of rapid clonal propagation, selection of imitative plants, somatic hybridization and genetic transformation.
而氨基硅烷磁性纳米颗粒和壳聚糖磁性纳米颗粒转化拟南芥原生质体未见GFP的表达。
But there were no GFP expression in arabidopsis protoplast with the amine silane magnetic nanoparticles and the chitosan magnetic nanoparticles transformation.
因而，原生质体制备和再生技术是禾长蠕孢菌转化与基因克隆技术的基础。
So it is a key step to establish an effective system of preparation and regeneration of HGE protoplasts.
因而，原生质体制备和再生技术是禾长蠕孢菌转化与基因克隆技术的基础。
So it is a key step to establish an effective system of preparation and regeneration of HGE protoplasts.
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